Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system - PubMed (original) (raw)

Interrogation of phosphor-specific interaction on a high-throughput label-free optical biosensor system-Epic system

Meng Wu et al. J Recept Signal Transduct Res. 2009.

Abstract

The Epic system, a high-throughput label-free optical biosensor system, is applied for the biochemical interrogation of phosphor-specific interactions of the 14-3-3 protein and its substrates. It has shown the capability not only for high-throughput characterization of binding rank and affinity but also for the exploration of potential interacting kinases for the substrates. A perspective of biochemical applications for diagnostics and biomarker discovery, as well as cell-based applications for endogenous receptors and viral infection characterization, are also provided.

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