Involvement of the membrane-cytoskeleton in development of epithelial cell polarity - PubMed (original) (raw)
Affiliations
- PMID: 1966328
Review
Involvement of the membrane-cytoskeleton in development of epithelial cell polarity
W J Nelson et al. Semin Cell Biol. 1990 Oct.
Abstract
The generation of cell surface polarity in transporting epithelial cells occurs in three distinct stages that involve cell-cell recognition and adhesion, cell surface remodelling to form biochemically and functionally distinct cell surface domains, and development of vectorial function. A widely used model system to study mechanisms involved in these stages is the Madin-Darby canine kidney (MDCK) cell line. Under appropriate growth conditions, MDCK cells develop in similar stages into polarized, multicellular epithelial structures. Analysis of membrane-cytoskeletal proteins ankyrin and fodrin during development of MDCK cell surface polarity shows that they gradually assemble into an insoluble protein complex on the basal-lateral membrane domain upon cell-cell adhesion, concomitantly with the redistribution of Na+,K(+)-ATPase, a marker protein of the basal-lateral membrane. Biochemical analysis shows that ankyrin, fodrin occur in a complex with Na+,K(+)-ATPase and the cell adhesion molecule uvomorulin in MDCK cells. A model is presented in which assembly of membrane-cytoskeletal complexes at sites of uvomorulin-induced cell-cell contact causes a remodelling of the cell surface distribution of specific membrane proteins which, in turn, contributes to the generation of epithelial cell surface polarity.
Similar articles
- Role of the membrane-cytoskeleton in the spatial organization of the Na,K-ATPase in polarized epithelial cells.
Nelson WJ, Hammerton RW, McNeill H. Nelson WJ, et al. Soc Gen Physiol Ser. 1991;46:77-87. Soc Gen Physiol Ser. 1991. PMID: 1653995 Review. - Distinguishing roles of the membrane-cytoskeleton and cadherin mediated cell-cell adhesion in generating different Na+,K(+)-ATPase distributions in polarized epithelia.
Marrs JA, Napolitano EW, Murphy-Erdosh C, Mays RW, Reichardt LF, Nelson WJ. Marrs JA, et al. J Cell Biol. 1993 Oct;123(1):149-64. doi: 10.1083/jcb.123.1.149. J Cell Biol. 1993. PMID: 8408194 Free PMC article. - High levels of E-/P-cadherin: correlation with decreased apical polarity of Na/K ATPase in bovine RPE cells in situ.
Burke JM, Cao F, Irving PE. Burke JM, et al. Invest Ophthalmol Vis Sci. 2000 Jun;41(7):1945-52. Invest Ophthalmol Vis Sci. 2000. PMID: 10845621 - Cytoskeleton functions in membrane traffic in polarized epithelial cells.
Nelson WJ. Nelson WJ. Semin Cell Biol. 1991 Dec;2(6):375-85. Semin Cell Biol. 1991. PMID: 1813027 Review.
Cited by
- The mechanical influence of densification on epithelial architecture.
Cammarota C, Dawney NS, Bellomio PM, Jüng M, Fletcher AG, Finegan TM, Bergstralh DT. Cammarota C, et al. PLoS Comput Biol. 2024 Apr 1;20(4):e1012001. doi: 10.1371/journal.pcbi.1012001. eCollection 2024 Apr. PLoS Comput Biol. 2024. PMID: 38557605 Free PMC article. - The Role of Nonerythroid Spectrin _α_II in Cancer.
Ackermann A, Brieger A. Ackermann A, et al. J Oncol. 2019 May 2;2019:7079604. doi: 10.1155/2019/7079604. eCollection 2019. J Oncol. 2019. PMID: 31186638 Free PMC article. Review. - Downregulation of SPTAN1 is related to MLH1 deficiency and metastasis in colorectal cancer.
Ackermann A, Schrecker C, Bon D, Friedrichs N, Bankov K, Wild P, Plotz G, Zeuzem S, Herrmann E, Hansmann ML, Brieger A. Ackermann A, et al. PLoS One. 2019 Mar 11;14(3):e0213411. doi: 10.1371/journal.pone.0213411. eCollection 2019. PLoS One. 2019. PMID: 30856214 Free PMC article. - Ankyrin-G palmitoylation and βII-spectrin binding to phosphoinositide lipids drive lateral membrane assembly.
He M, Abdi KM, Bennett V. He M, et al. J Cell Biol. 2014 Jul 21;206(2):273-88. doi: 10.1083/jcb.201401016. J Cell Biol. 2014. PMID: 25049274 Free PMC article. - Involvement of actinin-4 in the recruitment of JRAB/MICAL-L2 to cell-cell junctions and the formation of functional tight junctions.
Nakatsuji H, Nishimura N, Yamamura R, Kanayama HO, Sasaki T. Nakatsuji H, et al. Mol Cell Biol. 2008 May;28(10):3324-35. doi: 10.1128/MCB.00144-08. Epub 2008 Mar 10. Mol Cell Biol. 2008. PMID: 18332111 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources