Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max - PubMed (original) (raw)

Partial purification and characterization of a DNA helicase from chloroplasts of Glycine max

G C Cannon et al. Plant Mol Biol. 1990 Sep.

Abstract

A DNA helicase activity was detected in extracts of purified chloroplasts from the SB-1 cell line of Glycine max and partially purified by column chromatography on DEAE cellulose, phosphocellulose, and single-stranded DNA cellulose. The chloroplast helicase has a DNA-dependent ATPase activity, and its strand displacement activity is strictly dependent upon the presence of a nucleoside triphosphate and Mg2+ or Mn2+. Strand displacement activity does not require a free unannealed single-strand or replication fork-like structure.

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References

1. 1. Nucleic Acids Res. 1983 Mar 11;11(5):1523-36 - PubMed
2. 1. Proc Natl Acad Sci U S A. 1989 Jun;86(11):4156-60 - PubMed
3. 1. Plant Physiol. 1983 Jun;72(2):426-9 - PubMed
4. 1. J Biol Chem. 1983 Nov 25;258(22):14017-24 - PubMed
5. 1. J Biol Chem. 1986 Apr 5;261(10):4738-48 - PubMed

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