Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity - PubMed (original) (raw)

. 2009 Oct 9;27(43):5982-8.

doi: 10.1016/j.vaccine.2009.07.071. Epub 2009 Aug 7.

Daisuke Tokuhara, Tomonori Nochi, Hiroshi Yasuda, Mio Mejima, Shiho Kurokawa, Yuko Takahashi, Nobuhiro Kataoka, Ushio Nakanishi, Yukari Hagiwara, Kohtaro Fujihashi, Fumio Takaiwa, Hiroshi Kiyono

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Oral MucoRice expressing double-mutant cholera toxin A and B subunits induces toxin-specific neutralising immunity

Yoshikazu Yuki et al. Vaccine. 2009.

Abstract

Rice-expressed cholera toxin B (CTB) subunit is a cold-chain-free oral vaccine that effectively induces enterotoxin-neutralising immunity. We created another rice-based vaccine, MucoRice, expressing nontoxic double-mutant cholera toxin (dmCT) with CTA and CTB subunits. Western-blot analysis suggested that MucoRice-dmCT had the shape of a multicomponent vaccine. Oral administration of MucoRice-dmCT induced CTB- but not CTA-specific serum IgG and mucosal IgA antibodies, generating protective immunity against cholera toxin without inducing rice-protein-specific antibody responses. The potency of MucoRice-dmCT was equal to that of MucoRice-CTB vaccine. MucoRice has the potential to be used as a safe multicomponent vaccine expression system.

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