Antibodies to PB1-F2 protein are induced in response to influenza A virus infection - PubMed (original) (raw)

Antibodies to PB1-F2 protein are induced in response to influenza A virus infection

Ingrid Krejnusová et al. Arch Virol. 2009.

Abstract

PB1-F2 is a small influenza A virus (IAV) protein encoded by an alternative (+1) reading frame of the PB1 gene. While dispensable for IAV replication in cultured cells, PB1-F2 has been implicated in IAV pathogenicity. To better understand PB1-F2 expression in vivo and its immunogenicity, we analyzed anti-PB1-F2 antibodies (Abs) in sera of mice infected intranasally (i.n.) with A/PR/8/34 (H1N1) virus and human acute and convalescent sera collected from the influenza H3N2 winter 2003-2004 epidemic. We explored a number of methods for detecting anti-PB1-F2 Abs, finding that PB1-F2-specific Abs could clearly be detected via immunoprecipitation or immunofluorescence assays using both immune mouse and human convalescent sera. Importantly, paired human sera exhibited similar increases in HI titers and PB1-F2-specific Abs. This study indicates that PB1-F2 is expressed in sufficient quantities in mice and humans infected with IAV to elicit an Ab response, supporting the biological relevance of this intriguing accessory protein.

PubMed Disclaimer

Figures

Fig. 1

Fig. 1

WB analysis of human paired sera for PB1-F2. Acute (a) and convalescent (b) human sera, MAb AG55, positive control (PC), normal mouse serum, negative control (NC)

Fig. 2

Fig. 2

Immunoprecipitation analysis of immune mouse sera and human paired sera for PB1-F2-specific Abs. Immune mouse sera (1-10), human paired sera (4-20, acute (a), convalescent (b)), immune mouse serum to PB1-F2, positive control (PC), normal mouse serum, negative control (NC)

Fig. 3

Fig. 3

Immunofluorescence analysis of immune mouse sera (e, f, g, h) and human convalescent sera (i, j, k, l) for PB1-F2-specific Abs. MDCK cells infected with CR19 and detected with MAb TW2.3 (a), MAb AG55 (c), immune mouse sera (e, g), and human convalescent sera (i, k), respectively. MDCK cells infected with VV-PB1-F2 and detected with MAb TW2.3 (b), MAb AG55 (d), mouse immune sera (f, h), and human convalescent sera (j, l), respectively Table 1

Similar articles

Cited by

References

    1. Chen W, Calvo PA, Malide D, Gibbs J, Schubert U, Bacik I, Basta S, O’Neill R, Schickli J, Palese P, Henklein P, Bennink JR, Yewdell JW. A novel influenza A virus mitochondrial protein that induces cell death. Nat Med. 2001;7:1306–1312. - PubMed
    1. Pančuchárová H, Russ G. PB1-F2 gene in influenza A viruses of different hemagglutinin subtype. Acta Virol. 2006;50:269–272. - PubMed
    1. Gocnikova H, Russ G. Influenza a virus PB1-F2 protein. Acta Virol. 2007;51:101–108. - PubMed
    1. Gibbs JS, Malide D, Hornung F, Bennink JR, Yewdell JW. The influenza A virus PB1-F2 protein targets the inner mitochondrial membrane via a predicted basic amphipathic helix that disrupts mitochondrial function. J Virol. 2003;77:7214–7224. - PMC - PubMed
    1. Yamada H, Chounan R, Higashi Y, Kurihara N, Kido H. Mitochondrial targeting sequence of the influenza A virus PB1-F2 protein and its function in mitochondria. FEBS Lett. 2004;578:331–336. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources