Detection of hepatitis C viral sequences in non-A, non-B hepatitis - PubMed (original) (raw)

Detection of hepatitis C viral sequences in non-A, non-B hepatitis

A J Weiner et al. Lancet. 1990.

Abstract

The role of hepatitis C virus (HCV) in post-transfusion non-A, non-B hepatitis (NANBH) was investigated by analysing clinical samples for both HCV RNA by cDNA/polymerase chain reaction and antibodies against C100-3 by radioimmunoassay. Of fifteen chronic NANBH patients and one patient with chronic cryptogenic liver disease, ten were positive for anti-C100-3 and seven of the ten had viral sequences in their livers. However, two patients negative for anti-C100-3 also had substantial levels of HCV RNA in their livers. In acute post-transfusion NANBH (one surgical patient and two experimentally infected chimpanzees), HCV RNA was detected in the absence of anti-C100-3. In addition, infectious plasma from a seronegative patient with acute post-transfusion NANBH and a seronegative pool of plasma from a chimpanzee with chronic post-transfusion NANBH had high levels of HCV. These findings show that anti-C100-3-positive patients with chronic post-transfusion NANBH are likely to be viraemic; confirm that antibodies to C100-3 are a marker for infectivity; and suggest that the prevalence of HCV infections may be underestimated from the frequency of antibodies to C100-3 alone.

PubMed Disclaimer

Comment in

Detection of serum hepatitis C virus RNA.
Kaneko S, Unoura M, Kobayashi K, Kuno K, Murakami S, Hattori N. Kaneko S, et al. Lancet. 1990 Apr 21;335(8695):976. doi: 10.1016/0140-6736(90)91042-9. Lancet. 1990. PMID: 1970049 No abstract available.

Cited by

Use of a signature nucleotide sequence of hepatitis C virus for detection of viral RNA in human serum and plasma.
Cha TA, Kolberg J, Irvine B, Stempien M, Beall E, Yano M, Choo QL, Houghton M, Kuo G, Han JH, et al. Cha TA, et al. J Clin Microbiol. 1991 Nov;29(11):2528-34. doi: 10.1128/jcm.29.11.2528-2534.1991. J Clin Microbiol. 1991. PMID: 1663510 Free PMC article.
Detection of hepatitis C virus RNA in sera and liver tissues of non-A, non-B hepatitis patients using the polymerase chain reaction.
Ohkoshi S, Kato N, Kinoshita T, Hijikata M, Ohtsuyama Y, Okazaki N, Ohkura H, Hirohashi S, Honma A, Ozaki T, et al. Ohkoshi S, et al. Jpn J Cancer Res. 1990 Sep;81(9):862-5. doi: 10.1111/j.1349-7006.1990.tb02658.x. Jpn J Cancer Res. 1990. PMID: 2172195 Free PMC article.
Detection of hepatitis C virus ribonucleic acid in the serum by amplification with polymerase chain reaction.
Kato N, Yokosuka O, Omata M, Hosoda K, Ohto M. Kato N, et al. J Clin Invest. 1990 Nov;86(5):1764-7. doi: 10.1172/JCI114903. J Clin Invest. 1990. PMID: 2173727 Free PMC article.
Effects of anticoagulants and storage of blood samples on efficacy of the polymerase chain reaction assay for hepatitis C virus.
Wang JT, Wang TH, Sheu JC, Lin SM, Lin JT, Chen DS. Wang JT, et al. J Clin Microbiol. 1992 Mar;30(3):750-3. doi: 10.1128/jcm.30.3.750-753.1992. J Clin Microbiol. 1992. PMID: 1313053 Free PMC article.
Evidence for in vitro replication of hepatitis C virus genome in a human T-cell line.
Shimizu YK, Iwamoto A, Hijikata M, Purcell RH, Yoshikura H. Shimizu YK, et al. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5477-81. doi: 10.1073/pnas.89.12.5477. Proc Natl Acad Sci U S A. 1992. PMID: 1319062 Free PMC article.

Detection of hepatitis C viral sequences in non-A, non-B hepatitis - PubMed (original) (raw)