Site specific protein labeling by enzymatic posttranslational modification - PubMed (original) (raw)
Review
. 2009 Sep 7;7(17):3361-71.
doi: 10.1039/b908687k. Epub 2009 Jul 28.
Affiliations
- PMID: 19675886
- DOI: 10.1039/b908687k
Review
Site specific protein labeling by enzymatic posttranslational modification
Murat Sunbul et al. Org Biomol Chem. 2009.
Abstract
Site specific protein labeling plays a key role in elucidating the function of the proteins at the molecular level by revealing their locations in the cell, their interaction networks with other cellular components and the dynamic mechanisms of their bio-generation, trafficking and degradation in response to regulatory signals in a biological system. Site specific protein labeling is, in essence, artificial modification of proteins with new chemical entities at the posttranslational stage. Based on the analogy between protein labeling and protein posttranslational modification, enzymatic tools have been developed for site specific and efficient labeling of target proteins with chemical probes of diverse structures and functionalities. This perspective surveys a number of protein labeling methods based on the application of protein posttranslational modification enzymes.
Similar articles
- Labeling proteins with small molecules by site-specific posttranslational modification.
Yin J, Liu F, Li X, Walsh CT. Yin J, et al. J Am Chem Soc. 2004 Jun 30;126(25):7754-5. doi: 10.1021/ja047749k. J Am Chem Soc. 2004. PMID: 15212504 - Site-specific protein modification: advances and applications.
Foley TL, Burkart MD. Foley TL, et al. Curr Opin Chem Biol. 2007 Feb;11(1):12-9. doi: 10.1016/j.cbpa.2006.11.036. Epub 2006 Dec 26. Curr Opin Chem Biol. 2007. PMID: 17189712 Review. - Chemical probes shed light on protein function.
O'Hare HM, Johnsson K, Gautier A. O'Hare HM, et al. Curr Opin Struct Biol. 2007 Aug;17(4):488-94. doi: 10.1016/j.sbi.2007.07.005. Epub 2007 Sep 11. Curr Opin Struct Biol. 2007. PMID: 17851069 Review. - Site-specific labeling of proteins with small molecules in live cells.
Chen I, Ting AY. Chen I, et al. Curr Opin Biotechnol. 2005 Feb;16(1):35-40. doi: 10.1016/j.copbio.2004.12.003. Curr Opin Biotechnol. 2005. PMID: 15722013 Review. - Probing protein function by chemical modification.
Wu YW, Goody RS. Wu YW, et al. J Pept Sci. 2010 Oct;16(10):514-23. doi: 10.1002/psc.1287. J Pept Sci. 2010. PMID: 20814888 Review.
Cited by
- Combined Application of Orthogonal Sortases and Depsipeptide Substrates for Dual Protein Labeling.
Morgan HE, Arnott ZLP, Kamiński TP, Turnbull WB, Webb ME. Morgan HE, et al. Bioconjug Chem. 2022 Dec 21;33(12):2341-2347. doi: 10.1021/acs.bioconjchem.2c00411. Epub 2022 Nov 10. Bioconjug Chem. 2022. PMID: 36356167 Free PMC article. - Generation of Peptides for Highly Efficient Proximity Utilizing Site-Specific Biotinylation in Cells.
Kulyyassov A, Ramankulov Y, Ogryzko V. Kulyyassov A, et al. Life (Basel). 2022 Feb 16;12(2):300. doi: 10.3390/life12020300. Life (Basel). 2022. PMID: 35207587 Free PMC article. - Imaging VIPER-labeled Cellular Proteins by Correlative Light and Electron Microscopy.
Doh JK, Chang YH, Enns CA, Lόpez CS, Beatty KE. Doh JK, et al. Bio Protoc. 2019 Nov 5;9(21):e3414. doi: 10.21769/BioProtoc.3414. eCollection 2019 Nov 5. Bio Protoc. 2019. PMID: 33654913 Free PMC article. - Recent advances of antibody drug conjugates for clinical applications.
Zhao P, Zhang Y, Li W, Jeanty C, Xiang G, Dong Y. Zhao P, et al. Acta Pharm Sin B. 2020 Sep;10(9):1589-1600. doi: 10.1016/j.apsb.2020.04.012. Epub 2020 Apr 24. Acta Pharm Sin B. 2020. PMID: 33088681 Free PMC article. Review. - Antibody-Drug Conjugates for Cancer Therapy.
Hafeez U, Parakh S, Gan HK, Scott AM. Hafeez U, et al. Molecules. 2020 Oct 16;25(20):4764. doi: 10.3390/molecules25204764. Molecules. 2020. PMID: 33081383 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources