Two-photon excitation STED microscopy - PubMed (original) (raw)
. 2009 Aug 17;17(17):14567-73.
doi: 10.1364/oe.17.014567.
Affiliations
- PMID: 19687936
- DOI: 10.1364/oe.17.014567
Free article
Two-photon excitation STED microscopy
Gael Moneron et al. Opt Express. 2009.
Free article
Abstract
We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanoparticles and the immunostained transcription regulator NF kappaB in mammalian cell nuclei exhibit resolutions of <50 nm and approximately 70 nm in the focal plane, respectively, corresponding to a 4-5.4-fold improvement over the diffraction barrier.
(c) 2009 Optical Society of America
Similar articles
- STED microscopy with continuous wave beams.
Willig KI, Harke B, Medda R, Hell SW. Willig KI, et al. Nat Methods. 2007 Nov;4(11):915-8. doi: 10.1038/nmeth1108. Epub 2007 Oct 21. Nat Methods. 2007. PMID: 17952088 - Fast scanning STED and two-photon fluorescence excitation microscopy with continuous wave beam.
Bianchini P, Diaspro A. Bianchini P, et al. J Microsc. 2012 Mar;245(3):225-8. doi: 10.1111/j.1365-2818.2011.03577.x. Epub 2011 Dec 15. J Microsc. 2012. PMID: 22171566 - Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach MA, Keller J, Schönle A, Kamin D, Westphal V, Rizzoli SO, Hell SW. Lauterbach MA, et al. J Biophotonics. 2010 Jul;3(7):417-24. doi: 10.1002/jbio.201000038. J Biophotonics. 2010. PMID: 20379984 - Molecular photobleaching kinetics of Rhodamine 6G by one- and two-photon induced confocal fluorescence microscopy.
Eggeling C, Volkmer A, Seidel CA. Eggeling C, et al. Chemphyschem. 2005 May;6(5):791-804. doi: 10.1002/cphc.200400509. Chemphyschem. 2005. PMID: 15884061 Review. - Advances in Two-Photon Imaging in Plants.
Mizuta Y. Mizuta Y. Plant Cell Physiol. 2021 Nov 10;62(8):1224-1230. doi: 10.1093/pcp/pcab062. Plant Cell Physiol. 2021. PMID: 34019083 Free PMC article. Review.
Cited by
- The ATTO 565 Dye and Its Applications in Microscopy.
Wu Y, Williams RM. Wu Y, et al. Molecules. 2024 Sep 6;29(17):4243. doi: 10.3390/molecules29174243. Molecules. 2024. PMID: 39275091 Free PMC article. Review. - Multiplane differential saturated excitation microscopy using varifocal lenses.
Luo CH, Vyas S, Huang KY, Chu SW, Luo Y. Luo CH, et al. Biomed Opt Express. 2024 Jan 24;15(2):953-964. doi: 10.1364/BOE.504807. eCollection 2024 Feb 1. Biomed Opt Express. 2024. PMID: 38404334 Free PMC article. - All-synchronized picosecond pulses and time-gated detection improve the spatial resolution of two-photon STED microscopy in brain tissue imaging.
Ishii H, Otomo K, Chang CP, Yamasaki M, Watanabe M, Yokoyama H, Nemoto T. Ishii H, et al. PLoS One. 2023 Aug 24;18(8):e0290550. doi: 10.1371/journal.pone.0290550. eCollection 2023. PLoS One. 2023. PMID: 37616194 Free PMC article. - Advanced imaging techniques for tracking drug dynamics at the subcellular level.
Zhang C, Tian Z, Chen R, Rowan F, Qiu K, Sun Y, Guan JL, Diao J. Zhang C, et al. Adv Drug Deliv Rev. 2023 Aug;199:114978. doi: 10.1016/j.addr.2023.114978. Epub 2023 Jun 28. Adv Drug Deliv Rev. 2023. PMID: 37385544 Free PMC article. Review. - Improving two-photon excitation microscopy for sharper and faster biological imaging.
Otomo K, Ishii H, Nemoto T. Otomo K, et al. Biophys Physicobiol. 2023 Feb 8;20(1):e200009. doi: 10.2142/biophysico.bppb-v20.0009. eCollection 2023. Biophys Physicobiol. 2023. PMID: 37234851 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources