Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide - PubMed (original) (raw)

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

J Casey et al. Nucleic Acids Res. 1977.

Free PMC article

Abstract

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

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