Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide - PubMed (original) (raw)

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

J Casey et al. Nucleic Acids Res. 1977.

Free PMC article

Abstract

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

PubMed Disclaimer

Similar articles

• RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions.
  Chien YH, Davidson N. Chien YH, et al. Nucleic Acids Res. 1978 May;5(5):1627-37. doi: 10.1093/nar/5.5.1627. Nucleic Acids Res. 1978. PMID: 208059 Free PMC article.
• Renaturation kinetics and thermal stability of DNA in aqueous solutions of formamide and urea.
  Hutton JR. Hutton JR. Nucleic Acids Res. 1977 Oct;4(10):3537-55. doi: 10.1093/nar/4.10.3537. Nucleic Acids Res. 1977. PMID: 928068 Free PMC article.
• Reassociation of nucleic acids in solutions containing formamide.
  Blüthmann H, Brück D, Hübner L, Schöffski A. Blüthmann H, et al. Biochem Biophys Res Commun. 1973 Jan 4;50(1):91-7. doi: 10.1016/0006-291x(73)91068-1. Biochem Biophys Res Commun. 1973. PMID: 4567088 No abstract available.
• Hybridization and renaturation kinetics of nucleic acids.
  Wetmur JG. Wetmur JG. Annu Rev Biophys Bioeng. 1976;5:337-61. doi: 10.1146/annurev.bb.05.060176.002005. Annu Rev Biophys Bioeng. 1976. PMID: 7992 Review. No abstract available.

Cited by

• Multihormonal induction of hepatic alpha2u-globulin mRNA as measured by hybridization to complementary DNA.
  Kurtz DT, Feigelson P. Kurtz DT, et al. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4791-5. doi: 10.1073/pnas.74.11.4791. Proc Natl Acad Sci U S A. 1977. PMID: 73184 Free PMC article.
• Rapid sequence determination of late simian virus 40 16S mRNA leader by using inhibitors of reverse transcriptase.
  Bina-Stein M, Thoren M, Salzman N, Thomspon JA. Bina-Stein M, et al. Proc Natl Acad Sci U S A. 1979 Feb;76(2):731-5. doi: 10.1073/pnas.76.2.731. Proc Natl Acad Sci U S A. 1979. PMID: 85304 Free PMC article.
• Histological aspects of in situ hybridization. Detection of poly(A) nucleotide sequences in mouse liver sections as a model system.
  Jilbert AR, Burrell CJ, Gowans EJ, Rowland R. Jilbert AR, et al. Histochemistry. 1986;85(6):505-14. doi: 10.1007/BF00508433. Histochemistry. 1986. PMID: 3781890
• Preparative and analytical purification of DNA from agarose.
  Vogelstein B, Gillespie D. Vogelstein B, et al. Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9. doi: 10.1073/pnas.76.2.615. Proc Natl Acad Sci U S A. 1979. PMID: 284385 Free PMC article.
• "Transactivation" control signals in the promoter of the herpesvirus thymidine kinase gene.
  ElKareh A, Murphy AJ, Fichter T, Efstratiadis A, Silverstein S. ElKareh A, et al. Proc Natl Acad Sci U S A. 1985 Feb;82(4):1002-6. doi: 10.1073/pnas.82.4.1002. Proc Natl Acad Sci U S A. 1985. PMID: 2983322 Free PMC article.

References

1. 1. Anal Biochem. 1976 Jan;70(1):75-85 - PubMed
2. 1. J Mol Biol. 1962 Jul;5:109-18 - PubMed
3. 1. J Mol Biol. 1975 Mar 5;92(3):433-48 - PubMed
4. 1. Biochemistry. 1974 Dec 31;13(27):5467-73 - PubMed
5. 1. Biochemistry. 1972 Mar 28;11(7):1319-26 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Other Literature Sources

  • The Lens - Patent Citations Database