A vertebrate Polycomb response element governs segmentation of the posterior hindbrain - PubMed (original) (raw)
. 2009 Sep 4;138(5):885-97.
doi: 10.1016/j.cell.2009.08.020.
Affiliations
- PMID: 19737517
- DOI: 10.1016/j.cell.2009.08.020
Free article
A vertebrate Polycomb response element governs segmentation of the posterior hindbrain
Angela Sing et al. Cell. 2009.
Free article
Abstract
Chromatin remodeling by Polycomb group (PcG) and trithorax group (trxG) proteins regulates gene expression in all metazoans. Two major complexes, Polycomb repressive complexes 1 and 2 (PRC1 and PRC2), are thought to mediate PcG-dependent repression in flies and mammals. In Drosophila, PcG/trxG protein complexes are recruited by PcG/trxG response elements (PREs). However, it has been unclear how PcG/trxG are recruited in vertebrates. Here we have identified a vertebrate PRE, PRE-kr, that regulates expression of the mouse MafB/Kreisler gene. PRE-kr recruits PcG proteins in flies and mouse F9 cells and represses gene expression in a PcG/trxG-dependent manner. PRC1 and 2 bind to a minimal PRE-kr region, which can recruit stable PRC1 binding but only weak PRC2 binding when introduced ectopically, suggesting that PRC1 and 2 have different binding requirements. Thus, we provide evidence that similar to invertebrates, PREs act as entry sites for PcG/trxG chromatin remodeling in vertebrates.
Comment in
- Polycomb response elements in vertebrates.
Garrick D, De Gobbi M, Gibbons R, Higgs DR. Garrick D, et al. Epigenomics. 2009 Dec;1(2):231. doi: 10.2217/epi.09.36. Epigenomics. 2009. PMID: 22122700 No abstract available.
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