Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits - PubMed (original) (raw)
. 2009 Sep 4;138(5):911-22.
doi: 10.1016/j.cell.2009.06.045.
Affiliations
- PMID: 19737519
- DOI: 10.1016/j.cell.2009.06.045
Free article
Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits
Cornelia Ulbrich et al. Cell. 2009.
Free article
Abstract
The dynein-related AAA ATPase Rea1 is a preribosomal factor that triggers an unknown maturation step in 60S subunit biogenesis. Using electron microscopy, we show that Rea1's motor domain is docked to the pre-60S particle and its tail-like structure, harboring a metal ion-dependent adhesion site (MIDAS), protrudes from the preribosome. Typically, integrins utilize a MIDAS to bind extracellular ligands, an interaction that is strengthened under applied tensile force. Likewise, the Rea1 MIDAS binds the preribosomal factor Rsa4, which is located on the pre-60S subunit at a site that is contacted by the flexible Rea1 tail. The MIDAS-Rsa4 interaction is essential for ATP-dependent dissociation of a group of non-ribosomal factors from the pre-60S particle. Thus, Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit.
Comment in
- The Rea1 tadpole loses its tail.
Talkish J, Woolford JL Jr. Talkish J, et al. Cell. 2009 Sep 4;138(5):832-4. doi: 10.1016/j.cell.2009.08.019. Cell. 2009. PMID: 19737511
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