Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA - PubMed (original) (raw)

Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA

Gurdeep Rastogi et al. J Microbiol. 2009 Aug.

Abstract

A culture-independent molecular phylogenetic analysis was carried out to study the prokaryotic diversity in two soil samples collected from the subsurface (1.34 km depth) of the former Homestake gold mine, Lead, South Dakota, USA at two sites, the Ross shaft and number 6 Winze. Microbial community analyses were performed by cloning and sequencing of 16S rRNA genes retrieved directly from soil samples. Geochemical characterization of soils revealed high amount of toxic metals such as As, Cd, Co, Cr, Cu, Ni, Pb, Zn, and U at both the sites. Phylogenetic analyses showed that soil samples were predominantly composed of phylotypes related to phylum Proteobacteria. Other phyla detected in libraries were Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, Chlorobi, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Verrucomicrobia, and candidate divisions OP10 and TM7. The majority (>95%) of the phylotypes retrieved in the libraries were most closely related to environmental sequences from yet-uncultured bacteria representing a hitherto unidentified diversity. The archaeal communities at both the sites exhibited lower diversity and were most closely affiliated to uncultivated species within the Crenarchaeota. Results showed the existence of diverse microbial populations in deep subsurface environment of the Homestake gold mine. Statistical analyses demonstrated that each site harbored phylogenetically distinct microbial populations that were more diverse at Ross site compare to winze site.

PubMed Disclaimer

Cited by

The Response of a 16S Ribosomal RNA Gene Fragment Amplified Community to Lead, Zinc, and Copper Pollution in a Shanghai Field Trial.
Kou S, Vincent G, Gonzalez E, Pitre FE, Labrecque M, Brereton NJB. Kou S, et al. Front Microbiol. 2018 Mar 1;9:366. doi: 10.3389/fmicb.2018.00366. eCollection 2018. Front Microbiol. 2018. PMID: 29545788 Free PMC article.
Chemolithotrophy in the continental deep subsurface: Sanford Underground Research Facility (SURF), USA.
Osburn MR, LaRowe DE, Momper LM, Amend JP. Osburn MR, et al. Front Microbiol. 2014 Nov 12;5:610. doi: 10.3389/fmicb.2014.00610. eCollection 2014. Front Microbiol. 2014. PMID: 25429287 Free PMC article.
Metagenomics and Culture Dependent Insights into the Distribution of Firmicutes across Two Different Sample Types Located in the Black Hills Region of South Dakota, USA.
Govil T, Paste M, Samanta D, David A, Goh KM, Li X, Salem DR, Sani RK. Govil T, et al. Microorganisms. 2021 Jan 6;9(1):113. doi: 10.3390/microorganisms9010113. Microorganisms. 2021. PMID: 33418927 Free PMC article.
The prokaryotic community of a historically mining-impacted tropical stream sediment is as diverse as that from a pristine stream sediment.
Reis MP, Barbosa FA, Chartone-Souza E, Nascimento AM. Reis MP, et al. Extremophiles. 2013 Mar;17(2):301-9. doi: 10.1007/s00792-013-0517-9. Epub 2013 Feb 7. Extremophiles. 2013. PMID: 23389654
Analysis of unculturable bacterial communities in tea orchard soils based on nested PCR-DGGE.
Zhao J, Wu X, Nie C, Wu T, Dai W, Liu H, Yang R. Zhao J, et al. World J Microbiol Biotechnol. 2012 May;28(5):1967-79. doi: 10.1007/s11274-011-0999-5. Epub 2012 Jan 11. World J Microbiol Biotechnol. 2012. PMID: 22806018

References

1. Environ Microbiol. 2005 Dec;7(12):1967-84 - PubMed
1. Appl Environ Microbiol. 2004 Feb;70(2):1160-8 - PubMed
1. Appl Environ Microbiol. 2007 Aug;73(16):5261-7 - PubMed
1. Int J Syst Evol Microbiol. 2006 Jun;56(Pt 6):1209-1215 - PubMed
1. Int J Syst Evol Microbiol. 2005 May;55(Pt 3):1181-1186 - PubMed

Molecular analysis of prokaryotic diversity in the deep subsurface of the former Homestake gold mine, South Dakota, USA - PubMed (original) (raw)