Adenylyl cyclase type 5 contributes to corticostriatal plasticity and striatum-dependent learning - PubMed (original) (raw)

Endocannabinoid and cAMP modulation of corticostriatal plasticity in WT and AC5KO mice. A, Both genotypes show robust LTD in response to a 15 min bath application of the CB1 receptor agonist Win 55,212-2 (1 μ

m

; n = 5 WT and 8 AC5KO), which did not reverse during a 20 min bath application of the CB1 receptor antagonist AM251 [_N_-1-(2,4-dichlorophenyl)-5-(4-iodophenyl)-4-methyl-_N_-1-piperidinyl-1_H_-pyrazole-3-carboxamide] (10 μ

m

). B, Both genotypes show significant LTD in response to a 10 min bath application of the mGluR agonist DHPG (100 μ

m

; n = 8 WT and 11 AC5KO). Cells were voltage clamped at −50 mV throughout the recordings. C, A 1 min application of DHPG (100 μ

m

) in the presence of quinpirole (10 μ

m

) induced LTD in GFP-labeled D2 receptor-containing MSNs in WT but not AC5KO mice (n = 7 WT and 6 AC5KO). Cells were voltage clamped at −50 mV throughout the recordings. D, Including cAMP (1 m

m

) in the internal solution changes the type of plasticity induced by HFS in recordings from WT mice (n = 8 WT). The average plasticity observed in recordings from KO mice was not altered by including cAMP (1 m

m

) in the internal solution (n = 7 AC5KO). Summary data are presented as mean ± SEM.