Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria - PubMed (original) (raw)
. 2009 Dec;1793(12):1848-59.
doi: 10.1016/j.bbamcr.2009.10.002. Epub 2009 Oct 13.
Affiliations
- PMID: 19833151
- DOI: 10.1016/j.bbamcr.2009.10.002
Free article
Mitochondrial m-calpain plays a role in the release of truncated apoptosis-inducing factor from the mitochondria
Taku Ozaki et al. Biochim Biophys Acta. 2009 Dec.
Free article
Abstract
Calpains, calcium-dependent cysteine proteases, are involved in a variety of cellular processes. We have reported on the characteristics of mitochondrial mu-calpain and have shown that ERp57-associated mitochondrial mu-calpain cleaves the apoptosis-inducing factor (AIF) to a truncated form (tAIF). In addition, we found an unknown mitochondrial calpain. In this study, we identified and characterized this undescribed mitochondrial calpain in rat liver mitochondrial intermembrane space. The mitochondrial mu- and unknown calpains were separated by DEAE-Sepharose column chromatography. We immunoprecipitated the unknown calpain with anti-calpain small subunit and identified it as calpain 2 (m-calpain large subunit) by nanoflow-LC-MS/MS analysis and database searching. Because the identified mitochondrial calpain was stained with anti-m-calpain large subunit antibody, we named it mitochondrial m-calpain. The Ca(2+) dependency of mitochondrial m-calpain was similar to that of cytosolic m-calpain. Immunoprecipitation analyses showed that mitochondrial m-calpain is associated with a 75-kDa glucose-regulated protein, a member of the heat shock protein 70 family. We also investigated the involvement of mitochondrial m-calpain in the release of tAIF from mitochondria. Calpain inhibitor, PD150606, an anti-voltage-dependent anion channel (VDAC), and anti-Bax antibodies prevented the release of tAIF from mitochondria. In addition, we found that mitochondrial m-calpain truncated VDAC in Ca(2+)-dependent manner. This cleavage of VDAC promotes the mitochondrial accumulation of Bax and the release of tAIF from mitochondria. The accumulated Bax in mitochondrial outer membrane was co-immunoprecipitated with VDAC. Our results demonstrated that mitochondrial m-calpain plays a role in the release of tAIF from mitochondria by cleaving VDAC, and tAIF is released through VDAC-Bax pores.
Similar articles
- ERp57-associated mitochondrial μ-calpain truncates apoptosis-inducing factor.
Ozaki T, Yamashita T, Ishiguro S. Ozaki T, et al. Biochim Biophys Acta. 2008 Oct;1783(10):1955-63. doi: 10.1016/j.bbamcr.2008.05.011. Epub 2008 May 24. Biochim Biophys Acta. 2008. PMID: 18559257 - Characteristics of mitochondrial calpains.
Ozaki T, Tomita H, Tamai M, Ishiguro S. Ozaki T, et al. J Biochem. 2007 Sep;142(3):365-76. doi: 10.1093/jb/mvm143. Epub 2007 Jul 23. J Biochem. 2007. PMID: 17646173 - Ca²+-induced release of mitochondrial m-calpain from outer membrane with binding of calpain small subunit and Grp75.
Ozaki T, Yamashita T, Ishiguro S. Ozaki T, et al. Arch Biochem Biophys. 2011 Mar 15;507(2):254-61. doi: 10.1016/j.abb.2010.12.003. Epub 2010 Dec 9. Arch Biochem Biophys. 2011. PMID: 21145877 - The voltage-dependent anion channel (VDAC): function in intracellular signalling, cell life and cell death.
Shoshan-Barmatz V, Israelson A, Brdiczka D, Sheu SS. Shoshan-Barmatz V, et al. Curr Pharm Des. 2006;12(18):2249-70. doi: 10.2174/138161206777585111. Curr Pharm Des. 2006. PMID: 16787253 Review. - VDAC regulation: role of cytosolic proteins and mitochondrial lipids.
Rostovtseva TK, Bezrukov SM. Rostovtseva TK, et al. J Bioenerg Biomembr. 2008 Jun;40(3):163-70. doi: 10.1007/s10863-008-9145-y. J Bioenerg Biomembr. 2008. PMID: 18654841 Free PMC article. Review.
Cited by
- Proteolysis of mitochondrial calpain-13 in cerebral ischemia-reperfusion injury.
Chukai Y, Sudo T, Fukuda T, Tomita H, Sugano E, Ozaki T. Chukai Y, et al. Biochem Biophys Rep. 2024 Jul 1;39:101768. doi: 10.1016/j.bbrep.2024.101768. eCollection 2024 Sep. Biochem Biophys Rep. 2024. PMID: 39050013 Free PMC article. - Acute endoplasmic reticulum stress-induced mitochondria respiratory chain damage: The role of activated calpains.
Chen Q, Li L, Samidurai A, Thompson J, Hu Y, Willard B, Lesnefsky EJ. Chen Q, et al. FASEB J. 2024 Jan 31;38(2):e23404. doi: 10.1096/fj.202301158RR. FASEB J. 2024. PMID: 38197290 - Calpain: the regulatory point of myocardial ischemia-reperfusion injury.
Liu GY, Xie WL, Wang YT, Chen L, Xu ZZ, Lv Y, Wu QP. Liu GY, et al. Front Cardiovasc Med. 2023 Jun 29;10:1194402. doi: 10.3389/fcvm.2023.1194402. eCollection 2023. Front Cardiovasc Med. 2023. PMID: 37456811 Free PMC article. Review. - Novel dicarbonyl metabolic pathway via mitochondrial ES1 possessing glyoxalase III activity.
Ito G, Tatara Y, Itoh K, Yamada M, Yamashita T, Sakamoto K, Nozaki T, Ishida K, Wake Y, Kaneko T, Fukuda T, Sugano E, Tomita H, Ozaki T. Ito G, et al. BBA Adv. 2023 May 15;3:100092. doi: 10.1016/j.bbadva.2023.100092. eCollection 2023. BBA Adv. 2023. PMID: 37250100 Free PMC article. - Inhibition of extranodal NK/T-cell lymphoma by Chiauranib through an AIF-dependent pathway and its synergy with L-asparaginase.
Gao T, Huang J, Yin H, Huang J, Xie J, Zhou T, Fan W, Yang X, Gao G, Li Z. Gao T, et al. Cell Death Dis. 2023 May 9;14(5):316. doi: 10.1038/s41419-023-05833-w. Cell Death Dis. 2023. PMID: 37160920 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous