The S2 subunit of the spike glycoprotein of bovine coronavirus mediates membrane fusion in insect cells - PubMed (original) (raw)
The S2 subunit of the spike glycoprotein of bovine coronavirus mediates membrane fusion in insect cells
D W Yoo et al. Virology. 1991 Jan.
Abstract
The hemagglutinin/esterase (HE), spike precursor (S) and the S1 and S2 subunits of the spike precursor protein of bovine coronavirus were expressed in Spodoptera frugiperda (Sf9) cells, and the cell-fusing activity of each recombinant glycoprotein was examined. Extensive syncytia formation was observed in cells infected with the S2 recombinant but not with the HE or S1 recombinant baculoviruses. Fusion of Sf9 cells expressing the intact S protein precursor was evident after trypsin treatment. These results demonstrate that proteolytic cleavage of the S spike precursor is required for fusion induction and that the fusion is mediated by the S2 subunit. These observations may reflect the biological role of the S2 subunit in fusion-penetration during bovine coronavirus infection.
Similar articles
- Analysis of the S spike (peplomer) glycoprotein of bovine coronavirus synthesized in insect cells.
Yoo D, Parker MD, Babiuk LA. Yoo D, et al. Virology. 1990 Nov;179(1):121-8. doi: 10.1016/0042-6822(90)90281-u. Virology. 1990. PMID: 1699351 Free PMC article. - Fusion formation by the uncleaved spike protein of murine coronavirus JHMV variant cl-2.
Taguchi F. Taguchi F. J Virol. 1993 Mar;67(3):1195-202. doi: 10.1128/JVI.67.3.1195-1202.1993. J Virol. 1993. PMID: 8437210 Free PMC article. - Baculoviruses: high-level expression in insect cells.
Miller LK. Miller LK. Curr Opin Genet Dev. 1993 Feb;3(1):97-101. doi: 10.1016/s0959-437x(05)80348-x. Curr Opin Genet Dev. 1993. PMID: 8453280 Review. - Background paper. Coronavirus M and HE: two peculiar glycoproteins.
Rottier PJ. Rottier PJ. Adv Exp Med Biol. 1990;276:91-4. doi: 10.1007/978-1-4684-5823-7_13. Adv Exp Med Biol. 1990. PMID: 2103107 Review. No abstract available.
Cited by
- Molecular epidemiology of bovine coronavirus on the basis of comparative analyses of the S gene.
Liu L, Hägglund S, Hakhverdyan M, Alenius S, Larsen LE, Belák S. Liu L, et al. J Clin Microbiol. 2006 Mar;44(3):957-60. doi: 10.1128/JCM.44.3.957-960.2006. J Clin Microbiol. 2006. PMID: 16517883 Free PMC article. - Molecular analysis of S gene of spike glycoprotein of winter dysentery bovine coronavirus circulated in Korea during 2002-2003.
Jeong JH, Kim GY, Yoon SS, Park SJ, Kim YJ, Sung CM, Shin SS, Lee BJ, Kang MI, Park NY, Koh HB, Cho KO. Jeong JH, et al. Virus Res. 2005 Mar;108(1-2):207-12. doi: 10.1016/j.virusres.2004.07.003. Virus Res. 2005. PMID: 15681072 Free PMC article. - Structural characterization of the fusion-active complex of severe acute respiratory syndrome (SARS) coronavirus.
Ingallinella P, Bianchi E, Finotto M, Cantoni G, Eckert DM, Supekar VM, Bruckmann C, Carfi A, Pessi A. Ingallinella P, et al. Proc Natl Acad Sci U S A. 2004 Jun 8;101(23):8709-14. doi: 10.1073/pnas.0402753101. Epub 2004 May 25. Proc Natl Acad Sci U S A. 2004. PMID: 15161975 Free PMC article. - Pathogenesis of neurotropic murine coronavirus is multifactorial.
Phillips JM, Weiss SR. Phillips JM, et al. Trends Pharmacol Sci. 2011 Jan;32(1):2-7. doi: 10.1016/j.tips.2010.11.001. Epub 2010 Dec 7. Trends Pharmacol Sci. 2011. PMID: 21144598 Free PMC article. - The molecular biology of coronaviruses.
Lai MM, Cavanagh D. Lai MM, et al. Adv Virus Res. 1997;48:1-100. doi: 10.1016/S0065-3527(08)60286-9. Adv Virus Res. 1997. PMID: 9233431 Free PMC article. Review.
References
- Fan D., Sefton B. Cell. 1978;15:985–992. - PubMed
- Deregt D., Sabara M., Babiuk L.A. J. Gen. Virol. 1978;68:2863–2877. - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources