Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences - PubMed (original) (raw)
Novel mechanism for RNA maturation: the leader sequences of simian virus 40 mRNA are not transcribed adjacent to the coding sequences
Y Aloni et al. Proc Natl Acad Sci U S A. 1977 Sep.
Abstract
The 5'-terminal 100-200 ribonucleotides of late simian virus 40 (SV40) mRNAs are not transcribed immediately adjacent to their coding sequences. This conclusion is based on the following observations. The major late SV40 cytoplasmic RNA species, 16S and 19S, were purified from poly(A)-containing cytoplasmic RNA by hybridization to and elution from an SV40 DNA fragment that maps between 0.67 and 0.76. This fragment is remote from the DNA fragments that include the coding sequences. The RNA transcripts from the fragment located between 0.67 and 0.76 were found in abundance. Even though selected on oligo(dT)-cellulose columns, the 5'-terminal sequences did not contain poly(A) tails directly adjacent to their 3' termini. The 5' terminus of the 16S mRNA, as monitored by hybridization of the sequences adjacent to the "cap" structure, was found adjacent to the coding sequences when intact [3H]methyl-labeled RNA was hybridized with restriction fragments. However, after fragmentation, the methyl label of this same RNA hybridized with a fragment that is remote from the coding sequences and maps between 0.67 and 0.73. These results imply a novel mechanism for biosynthesis of SV40 mRNA.
Similar articles
- Mapping the spliced and unspliced late lytic SV40 RNAs.
Lai CJ, Dhar R, Khoury G. Lai CJ, et al. Cell. 1978 Aug;14(4):971-82. doi: 10.1016/0092-8674(78)90351-3. Cell. 1978. PMID: 210961 - Mapping of transcription sites of simian virus 40-specific late 16S and 19S mRNA by electron microscopy.
May E, Maizel JV, Salzman NP. May E, et al. Proc Natl Acad Sci U S A. 1977 Feb;74(2):496-500. doi: 10.1073/pnas.74.2.496. Proc Natl Acad Sci U S A. 1977. PMID: 191812 Free PMC article. - 5'-Terminal sequences and coding region of late simian virus 40 mRNAs are derived from noncontiguous segments of the viral genome.
Lavi S, Groner Y. Lavi S, et al. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5323-7. doi: 10.1073/pnas.74.12.5323. Proc Natl Acad Sci U S A. 1977. PMID: 202950 Free PMC article. - Synthesis and processing of simian virus 40-specific RNA in adenovirus-infected, simian virus 40-transformed human cells.
Flint SJ, Beltz GA, Linzer DI. Flint SJ, et al. J Mol Biol. 1983 Jun 25;167(2):335-59. doi: 10.1016/s0022-2836(83)80339-8. J Mol Biol. 1983. PMID: 6306259 - Splicing of viral mRNAs.
Aloni Y. Aloni Y. Prog Nucleic Acid Res Mol Biol. 1981;25:1-31. doi: 10.1016/s0079-6603(08)60481-x. Prog Nucleic Acid Res Mol Biol. 1981. PMID: 6261295 Review. No abstract available.
Cited by
- Principles and correction of 5'-splice site selection.
Malard F, Mackereth CD, Campagne S. Malard F, et al. RNA Biol. 2022 Jan;19(1):943-960. doi: 10.1080/15476286.2022.2100971. RNA Biol. 2022. PMID: 35866748 Free PMC article. Review. - Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA.
Vlak JM, Smith GE, Summers MD. Vlak JM, et al. J Virol. 1981 Dec;40(3):762-71. doi: 10.1128/JVI.40.3.762-771.1981. J Virol. 1981. PMID: 16789215 Free PMC article. - Viruses as model systems in cell biology.
Compans RW, Roberts PC. Compans RW, et al. Methods Cell Biol. 1994;43 Pt A:3-42. doi: 10.1016/s0091-679x(08)60596-8. Methods Cell Biol. 1994. PMID: 7823868 Free PMC article. Review. - Capped and polyadenylated low-molecular-weight RNA synthesized by vaccinia virus in vitro.
Paoletti E, Lipinskas BR, Panicali D. Paoletti E, et al. J Virol. 1980 Jan;33(1):208-19. doi: 10.1128/JVI.33.1.208-219.1980. J Virol. 1980. PMID: 7365867 Free PMC article. - Attenuation and modulation of mRNA secondary structure in a feedback control system regulating SV40 gene expression.
Aloni Y, Hay N. Aloni Y, et al. Mol Biol Rep. 1983 May;9(1-2):91-100. doi: 10.1007/BF00777479. Mol Biol Rep. 1983. PMID: 6310367
References
- Adv Virus Res. 1977;21:85-173 - PubMed
- Proc Natl Acad Sci U S A. 1977 Mar;74(3):827-31 - PubMed
- J Virol. 1976 Oct;20(1):324-9 - PubMed
- Proc Natl Acad Sci U S A. 1974 Feb;71(2):371-5 - PubMed
- J Virol. 1972 Aug;10(2):193-201 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous