A classification system for plasmids from enterococci and other Gram-positive bacteria - PubMed (original) (raw)
A classification system for plasmids from enterococci and other Gram-positive bacteria
L B Jensen et al. J Microbiol Methods. 2010 Jan.
Abstract
A classification system for plasmids isolated from enterococci and other Gram-positive bacteria was developed based on 111 published plasmid sequences from enterococci and other Gram-positive bacteria; mostly staphylococci. Based on PCR amplification of conserved areas of the replication initiating genes (rep), alignment of these sequences and using a cutoff value of 80% identity on both protein and DNA level, 19 replicon families (rep-families) were defined together with several unique sequences. The prevalence of these rep-families was tested on 79 enterococcal isolates from a collection of isolates of animal and human origin. Difference in prevalence of the designed rep-families were detected with rep(9) being most prevalent in Enterococcus faecalis and rep(2) in Enterococcus faecium. In 33% of the tested E. faecium and 32% of the tested E. faecalis no positive amplicons were detected. Furthermore, conjugation experiments were performed obtaining 30 transconjugants when selecting for antimicrobial resistance. Among them 19 gave no positive amplicons indicating presence of rep-families not tested for in this experimental setup.
Copyright 2009 Elsevier B.V. All rights reserved.
Similar articles
- VanA-type enterococci from humans, animals, and food: species distribution, population structure, Tn1546 typing and location, and virulence determinants.
Biavasco F, Foglia G, Paoletti C, Zandri G, Magi G, Guaglianone E, Sundsfjord A, Pruzzo C, Donelli G, Facinelli B. Biavasco F, et al. Appl Environ Microbiol. 2007 May;73(10):3307-19. doi: 10.1128/AEM.02239-06. Epub 2007 Mar 9. Appl Environ Microbiol. 2007. PMID: 17351100 Free PMC article. - Investigating the mobilome in clinically important lineages of Enterococcus faecium and Enterococcus faecalis.
Mikalsen T, Pedersen T, Willems R, Coque TM, Werner G, Sadowy E, van Schaik W, Jensen LB, Sundsfjord A, Hegstad K. Mikalsen T, et al. BMC Genomics. 2015 Apr 10;16:282. doi: 10.1186/s12864-015-1407-6. BMC Genomics. 2015. PMID: 25885771 Free PMC article. - Distribution and Transfer of Plasmid Replicon Families among Multidrug-Resistant Enterococcus faecalis and Enterococcus faecium from Poultry.
Cho S, McMillan EA, Barrett JB, Hiott LM, Woodley TA, House SL, Frye JG, Jackson CR. Cho S, et al. Microorganisms. 2022 Jun 17;10(6):1244. doi: 10.3390/microorganisms10061244. Microorganisms. 2022. PMID: 35744761 Free PMC article. - Presence of pRI1: a small cryptic mobilizable plasmid isolated from Enterococcus faecium of human and animal origin.
Garcia-Migura L, Hasman H, Jensen LB. Garcia-Migura L, et al. Curr Microbiol. 2009 Feb;58(2):95-100. doi: 10.1007/s00284-008-9266-x. Epub 2008 Oct 2. Curr Microbiol. 2009. PMID: 18830745 - Enterococci of animal origin and their significance for public health.
Hammerum AM. Hammerum AM. Clin Microbiol Infect. 2012 Jul;18(7):619-25. doi: 10.1111/j.1469-0691.2012.03829.x. Epub 2012 Apr 4. Clin Microbiol Infect. 2012. PMID: 22487203 Review.
Cited by
- Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in Enterococcus faecalis.
Michaelis C, Berger TMI, Kuhlmann K, Ghulam R, Petrowitsch L, Besora Vecino M, Gesslbauer B, Pavkov-Keller T, Keller W, Grohmann E. Michaelis C, et al. Front Mol Biosci. 2024 Feb 6;11:1268647. doi: 10.3389/fmolb.2024.1268647. eCollection 2024. Front Mol Biosci. 2024. PMID: 38380428 Free PMC article. - Genomic Analysis of a Community-Acquired Methicillin-Resistant Staphylococcus aureus Sequence Type 1 Associated with Caprine Mastitis.
Vasconcelos PC, Leite EL, Saraiva MMS, Ferrari RG, Cibulski SP, Silva NMV, Freitas Neto OC, Givisiez PEN, Vieira RFC, Oliveira CJB. Vasconcelos PC, et al. Pathogens. 2023 Dec 26;13(1):23. doi: 10.3390/pathogens13010023. Pathogens. 2023. PMID: 38251331 Free PMC article. - Characterization of a Tigecycline-, Linezolid- and Vancomycin-Resistant Clinical Enteroccoccus faecium Isolate, Carrying vanA and vanB Genes.
Wardal E, Żabicka D, Skalski T, Kubiak-Pulkowska J, Hryniewicz W, Sadowy E. Wardal E, et al. Infect Dis Ther. 2023 Nov;12(11):2545-2565. doi: 10.1007/s40121-023-00881-3. Epub 2023 Oct 11. Infect Dis Ther. 2023. PMID: 37821741 Free PMC article. - Phylogeny, Virulence, and Antimicrobial Resistance Gene Profiles of Enterococcus faecium Isolated from Australian Feedlot Cattle and Their Significance to Public and Environmental Health.
Messele YE, Trott DJ, Hasoon MF, Veltman T, McMeniman JP, Kidd SP, Petrovski KR, Low WY. Messele YE, et al. Antibiotics (Basel). 2023 Jun 28;12(7):1122. doi: 10.3390/antibiotics12071122. Antibiotics (Basel). 2023. PMID: 37508218 Free PMC article. - The Plasmidomic Landscape of Clinical Methicillin-Resistant Staphylococcus aureus Isolates from Malaysia.
Al-Trad EI, Chew CH, Che Hamzah AM, Suhaili Z, Rahman NIA, Ismail S, Puah SM, Chua KH, Kwong SM, Yeo CC. Al-Trad EI, et al. Antibiotics (Basel). 2023 Apr 9;12(4):733. doi: 10.3390/antibiotics12040733. Antibiotics (Basel). 2023. PMID: 37107095 Free PMC article.