Transcription complex disruption caused by a transition in chromatin structure - PubMed (original) (raw)

Transcription complex disruption caused by a transition in chromatin structure

G Almouzni et al. Mol Cell Biol. 1991 Feb.

Abstract

Chromatin structure is known to influence class III gene expression in vitro. We describe the active transcription of Xenopus class III genes following replication and assembly into chromatin by using Xenopus egg extracts. Changes in the structure of this active chromatin dependent on the presence of exogeneous Mg2+ ATP or on the addition of a mixture of histones H2A and H2B are shown to lead to the selective repression of Xenopus 5S RNA genes. Preexisting transcription complexes on 5S DNA are disrupted following the reorganization of a "disordered" histone-DNA complex into a structure consisting of physiologically spaced nucleosomes. Thus, we demonstrate that chromatin structural transitions can have dominant and specific effects on transcription.

PubMed Disclaimer

Similar articles

• Developmental regulation of two 5S ribosomal RNA genes.
  Wolffe AP, Brown DD. Wolffe AP, et al. Science. 1988 Sep 23;241(4873):1626-32. doi: 10.1126/science.241.4873.1626. Science. 1988. PMID: 3420414
• Histones H2A/H2B inhibit the interaction of transcription factor IIIA with the Xenopus borealis somatic 5S RNA gene in a nucleosome.
  Hayes JJ, Wolffe AP. Hayes JJ, et al. Proc Natl Acad Sci U S A. 1992 Feb 15;89(4):1229-33. doi: 10.1073/pnas.89.4.1229. Proc Natl Acad Sci U S A. 1992. PMID: 1741376 Free PMC article.
• Differential kinetics of transcription complex assembly distinguish oocyte and somatic 5S RNA genes of Xenopus.
  McBryant SJ, Gottesfeld JM. McBryant SJ, et al. Gene Expr. 1997;6(6):387-99. Gene Expr. 1997. PMID: 9495319 Free PMC article.
• Xenopus transcription factor IIIA and the 5S nucleosome: development of a useful in vitro system.
  Yang Z, Hayes JJ. Yang Z, et al. Biochem Cell Biol. 2003 Jun;81(3):177-84. doi: 10.1139/o03-043. Biochem Cell Biol. 2003. PMID: 12897852 Review.
• The role of transcription factors, chromatin structure and DNA replication in 5 S RNA gene regulation.
  Wolffe AP. Wolffe AP. J Cell Sci. 1994 Aug;107 ( Pt 8):2055-63. doi: 10.1242/jcs.107.8.2055. J Cell Sci. 1994. PMID: 7983167 Review.

Cited by

• Local nuclear to cytoplasmic ratio regulates chaperone-dependent H3 variant incorporation during zygotic genome activation.
  Bhatt AD, Brown MG, Wackford AB, Shindo Y, Amodeo AA. Bhatt AD, et al. bioRxiv [Preprint]. 2024 Jul 16:2024.07.15.603602. doi: 10.1101/2024.07.15.603602. bioRxiv. 2024. PMID: 39071352 Free PMC article. Preprint.
• Differential nuclear import sets the timing of protein access to the embryonic genome.
  Nguyen T, Costa EJ, Deibert T, Reyes J, Keber FC, Tomschik M, Stadlmeier M, Gupta M, Kumar CK, Cruz ER, Amodeo A, Gatlin JC, Wühr M. Nguyen T, et al. Nat Commun. 2022 Oct 6;13(1):5887. doi: 10.1038/s41467-022-33429-z. Nat Commun. 2022. PMID: 36202846 Free PMC article.
• The Nuclear-to-Cytoplasmic Ratio: Coupling DNA Content to Cell Size, Cell Cycle, and Biosynthetic Capacity.
  Balachandra S, Sarkar S, Amodeo AA. Balachandra S, et al. Annu Rev Genet. 2022 Nov 30;56:165-185. doi: 10.1146/annurev-genet-080320-030537. Epub 2022 Aug 17. Annu Rev Genet. 2022. PMID: 35977407 Free PMC article. Review.
• Histone concentration regulates the cell cycle and transcription in early development.
  Chari S, Wilky H, Govindan J, Amodeo AA. Chari S, et al. Development. 2019 Oct 4;146(19):dev177402. doi: 10.1242/dev.177402. Development. 2019. PMID: 31511251 Free PMC article.
• Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts.
  Wang WL, Shechter D. Wang WL, et al. Int J Dev Biol. 2016;60(7-8-9):315-320. doi: 10.1387/ijdb.160161ds. Int J Dev Biol. 2016. PMID: 27759158 Free PMC article.

References

1. 1. Mol Cell Biol. 1990 Jun;10(6):2863-73 - PubMed
2. 1. Nucleic Acids Res. 1990 Oct 11;18(19):5767-74 - PubMed
3. 1. Cell. 1987 Dec 24;51(6):1009-18 - PubMed
4. 1. EMBO J. 1988 Mar;7(3):665-72 - PubMed
5. 1. EMBO J. 1988 Apr;7(4):1071-9 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Taylor & Francis