Description of the novel perchlorate-reducing bacteria Dechlorobacter hydrogenophilus gen. nov., sp. nov.and Propionivibrio militaris, sp. nov - PubMed (original) (raw)

Description of the novel perchlorate-reducing bacteria Dechlorobacter hydrogenophilus gen. nov., sp. nov.and Propionivibrio militaris, sp. nov

J Cameron Thrash et al. Appl Microbiol Biotechnol. 2010 Mar.

Abstract

Novel dissimilatory perchlorate-reducing bacteria (DPRB) were isolated from enrichments conducted under conditions different from those of all previously described DPRB. Strain LT-1(T) was enriched using medium buffered at pH 6.6 with 2-(N-morpholino)ethanesulfonic acid (MES) and had only 95% 16S rRNA gene identity with its closest relative, Azonexus caeni. Strain MP(T) was enriched in the cathodic chamber of a perchlorate-reducing bioelectrical reactor (BER) and together with an additional strain, CR (99% 16S rRNA gene identity), had 97% 16S rRNA gene identity with Propionivibrio limicola. The use of perchlorate and other electron acceptors distinguished strains MP(T) and CR from P. limicola physiologically. Strain LT-1(T) had differences in electron donor utilization and optimum growth temperatures from A. caeni. Strains LT-1(T) and MP(T) are the first DPRB to be described in the Betaproteobacteria outside of the Dechloromonas and Azospira genera. On the basis of phylogenetic and physiological features, strain LT-1(T) represents a novel genus in the Rhodocyclaceae; strain MP(T) represents a novel species within the genus Propionivibrio. The names Dechlorobacter hydrogenophilus gen. nov., sp. nov and Propionivibrio militaris sp. nov. are proposed.

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Figures

Fig. 1

Fig. 1

SEM of strain MPT, scale bar = 1 µm

Fig. 2

Fig. 2

SEMs of strain LT-1T, scale bars = 125 nm

Fig. 3

Fig. 3

Growth curves of strain MPT (a) and strain LT-1T (b). Cell number increase was monitored with optical density (OD) at 600 nm. Closed circles OD, open squares [ClO4−], open triangles [Cl−], open diamonds, [ClO3−]; all concentrations in millimolar. Error bars represent the standard deviation of triplicate experiments

Fig. 4

Fig. 4

Bayesian 16S rRNA gene phylogenetic tree showing the position of strains MPT, CR, and LT-1T in the Rhodocylaceae. Scale bar represents 0.07 changes per position. Circles at the nodes indicate posterior probabilities >0.85 (closed) or <0.85 (open)

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