Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum - PubMed (original) (raw)
doi: 10.1136/gut.2009.191320. Epub 2009 Nov 18.
Yvonne Dörffel, Vera Loening-Baucke, Franz Theissig, Jens C Rückert, Mahmoud Ismail, Walter A Rau, Dagmar Gaschler, Michael Weizenegger, Sigmar Kühn, Johannes Schilling, Wolf V Dörffel
Affiliations
- PMID: 19926616
- DOI: 10.1136/gut.2009.191320
Acute appendicitis is characterised by local invasion with Fusobacterium nucleatum/necrophorum
Alexander Swidsinski et al. Gut. 2011 Jan.
Abstract
Background: Acute appendicitis is a local intestinal inflammation with unclear origin. The aim was to test whether bacteria in appendicitis differ in composition to bacteria found in caecal biopsies from healthy and disease controls.
Methods and patients: We investigated sections of 70 appendices using rRNA-based fluorescence in situ hybridisation. Four hundred caecal biopsies and 400 faecal samples from patients with inflammatory bowel disease and other conditions were used as controls. A set of 73 group-specific bacterial probes was applied for the study.
Results: The mucosal surface in catarrhal appendicitis showed characteristic lesions of single epithelial cells filled with a mixed bacterial population ('pinned cells') without ulceration of the surroundings. Bacteria deeply infiltrated the tissue in suppurative appendicitis. Fusobacteria (mainly Fusobacterium nucleatum and necrophorum) were a specific component of these epithelial and submucosal infiltrates in 62% of patients with proven appendicitis. The presence of Fusobacteria in mucosal lesions correlated positively with the severity of the appendicitis and was completely absent in caecal biopsies from healthy and disease controls. Main faecal microbiota represented by Bacteroides, Eubacterium rectale (Clostridium group XIVa), Faecalibacterium prausnitzii groups and Akkermansia muciniphila were inversely related to the severity of the disease. The occurrence of other bacterial groups within mucosal lesions of acute appendicitis was not related to the severity of the appendicitis. No Fusobacteria were found in rectal swabs of patients with acute appendicitis.
Conclusions: Local infection with Fusobacterium nucleatum/necrophorum is responsible for the majority of cases of acute appendicitis.
Comment in
- Local appendiceal dysbiosis: the missing link between the appendix and ulcerative colitis?
Roblin X, Neut C, Darfeuille-Michaud A, Colombel JF. Roblin X, et al. Gut. 2012 Apr;61(4):635-6. doi: 10.1136/gutjnl-2011-300576. Epub 2011 Jul 7. Gut. 2012. PMID: 21737859 No abstract available.
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