The Mcm complex: unwinding the mechanism of a replicative helicase - PubMed (original) (raw)

Evolutionary relationship among the Mcm families. The indicated Mcm sequences were aligned using ClustalX (141), and the phylogenic relationships among them were drawn as an unrooted tree using the Drawtree interface of PHYLIP 3.67 (

http://mobyle.pasteur.fr/cgi-bin/MobylePortal/portal.py?form=drawtree

). It should be noted that although the archaeal Mcm proteins are frequently shown to be most closely related to Mcm4 (42, 129), our more extensive analysis using a larger number of Mcm sequences failed to duplicate these results. Mcm genes from the following organisms were used: Homo sapiens (Hs), Danio rerio (Dr), Drosophila melanogaster (Dm), Caenorhabditis elegans (Ce), Schizosaccharomyces pombe (Sp), Saccharomyces cerevisiae (Sc), Arabidopsis thaliana (At), Oryza sativa (Os), Dictyostelium discoideum (Dd), Tetrahymena thermophila (Tt), Giardia lamblia (Gl), Sulfolobus solfataricus (Sso), Methanobacterium thermoautotrophicum (Mth), “_Nanoarchaeum equitans_” (Neq), and “_Korarchaeum cryptofilum_” (Kc). Note that although bacteria lack the Mcm proteins, several phages possess an _MCM_-like gene (e.g., a Bacillus cereus prophage [173], the “_Haloarcula sinaiiensis_” archaephage HSTV-1 [R. Hendrix, personal communication], and the archaeal virus BJ1 [E. Pagaling et al., unpublished data; see

http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=CAL92457

]); the BJ1 Mcm homologue has also been included in Fig. 1. GenBank accession numbers are as follows: AtMcm2, NP_175112.2; AtMcm3, NP_199440.1; AtMcm4, NP_179236.3; AtMcm5, NP_178812.1; AtMcm6, NP_680393.1; AtMcm7, NP_192115.1; AtMcm8, NP_187577.1; AtMcm9, NP_179021.2; BJ1 Mcm, YP_919062; CeMcm2, NP_001022416.1; CeMcm3, NC_003283.9; CeMcm4, NP_490962.1; CeMcm5, NP_497858.1; CeMcm6, NP_001023011.1; CeMcm7, NP_504199.1; DdMcm2, XP_637579.1; DdMcm3, NC_007089.3; DdMcm4, NC_007088.4; DdMcm5, NC_007092.2; DdMcm6, NC_007088.4; DdMcm7, NC_007090.2; DdMcm8, XP_639313.1; DdMcm9, XP_637904.1; DmMcm2, NP_477121.1; DmMcm3, NP_511048.2; DmMcm4, NP_477185.1; DmMcm5, NP_524308.2; DmMcm6, NP_511065.1; DmMcm7, NP_523984.1; DrMcm2, NP_775364.1; DrMcm3, NP_997732.1; DrMcm4, NP_944595.1; DrMcm5, NP_848523.2; DrMcm6, NP_001076318.1; DrMcm7, NP_997734.1; GlMcm2, EAA40971.1; GlMcm3, EAA36979.1; GlMcm4, EAA40854.1; GlMcm5, EAA38067.1; GlMcm6, EAA40537.1; GlMcm7, EAA40792.1; HsMcm2, NP_004517.2; HsMcm3, NP_002379.2; HsMcm4, NP_005905.2; HsMcm5, NP_006730.2; HsMcm6, NP_005906.2; HsMcm7, NP_005907.3; HsMcm8, NP_115874.3; HsMcm9, NP_694987.1; McmKc, ACB08098.1; MthMcm, AAB86236.1; Nanoarchaeum equitans Mcm, AAR39132.1; OsMcm2, NP_001067910.1; OsMcm3, NP_001055835.1; OsMcm4, AP004232.4; OsMcm5, NP_001048396.1; OsMcm6, NP_001054989.1; OsMcm7, NP_001067020.1; ScMcm2, NP_009530.1; ScMcm3, NP_010882.1; ScMcm4, NP_015344.1; ScMcm5, NP_013376.1; ScMcm6, NP_011314.2; ScMcm7, NP_009761.1; SpMcm2, NP_595477.1; SpMcm3, NP_587795.1; SpMcm4, NP_588004.2; SpMcm5, XP_001713071; SpMcm6, NP_596614.1; SpMcm7, NP_596545.1; SsoMcm, AAK41071.1; TtMcm2, XP_001009217.2; TtMcm3, XP_001012838.1; TtMcm4, XP_001018113.1; TtMcm5, XP_001007780.1; TtMcm6, XP_001013258.1; TtMcm7, XP_001008253.2; TtMcm8, XP_001012433.2; TtMcm9, XP_001032062.2.