Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis - PubMed (original) (raw)
. 2010 Jan;283(1):99-110.
doi: 10.1007/s00438-009-0500-4. Epub 2009 Nov 28.
Affiliations
- PMID: 19946786
- DOI: 10.1007/s00438-009-0500-4
Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis
Yasunori Sasakura et al. Mol Genet Genomics. 2010 Jan.
Abstract
Epigenetic regulation of genes plays a critical role in achieving proper gene expression during development, and it has been reported that epigenetic modifications are associated with transposon silencing in many organisms. Here, we report a type of epigenetic gene silencing, maternal gfp/gene silencing (MGS), in the basal chordate Ciona intestinalis. A transgenic line of Ciona, Tg[MiTFr3dTPOG]45 (abbreviated as Tg45), which was created with the Minos transposon, has a tandemly arrayed insertion of gfp in the promoter region of Ci-CesA. Progeny of Tg45 showed a reduced level of GFP expression when eggs of Tg45 were fertilized with sperm of other gfp transgenic lines. Although the genotype is the same, animals developed from Tg45 sperm and the eggs of other transgenic lines did not exhibit this phenomenon, suggesting the involvement of a maternal cytoplasmic factor that influences GFP expression. The silencing starts during oogenesis and continues after fertilization without any tissue specificity. We found that post-transcriptional degradation of the gfp mRNA is involved in MGS.
Similar articles
- Transposon-mediated targeted and specific knockdown of maternally expressed transcripts in the ascidian Ciona intestinalis.
Iitsuka T, Mita K, Hozumi A, Hamada M, Satoh N, Sasakura Y. Iitsuka T, et al. Sci Rep. 2014 May 23;4:5050. doi: 10.1038/srep05050. Sci Rep. 2014. PMID: 24854849 Free PMC article. - Enhancer detection in the ascidian Ciona intestinalis with transposase-expressing lines of Minos.
Sasakura Y, Konno A, Mizuno K, Satoh N, Inaba K. Sasakura Y, et al. Dev Dyn. 2008 Jan;237(1):39-50. doi: 10.1002/dvdy.21333. Dev Dyn. 2008. PMID: 17948255 - High-throughput enhancer trap by remobilization of transposon Minos in Ciona intestinalis.
Awazu S, Matsuoka T, Inaba K, Satoh N, Sasakura Y. Awazu S, et al. Genesis. 2007 May;45(5):307-17. doi: 10.1002/dvg.20290. Genesis. 2007. PMID: 17464954 - Germline transgenesis and insertional mutagenesis in the ascidian Ciona intestinalis.
Sasakura Y. Sasakura Y. Dev Dyn. 2007 Jul;236(7):1758-67. doi: 10.1002/dvdy.21111. Dev Dyn. 2007. PMID: 17342755 Review. - Transposon mediated transgenesis in a marine invertebrate chordate: Ciona intestinalis.
Sasakura Y, Oogai Y, Matsuoka T, Satoh N, Awazu S. Sasakura Y, et al. Genome Biol. 2007;8 Suppl 1(Suppl 1):S3. doi: 10.1186/gb-2007-8-s1-s3. Genome Biol. 2007. PMID: 18047695 Free PMC article. Review.
Cited by
- Pax3/7 regulates neural tube closure and patterning in a non-vertebrate chordate.
Kim K, Orvis J, Stolfi A. Kim K, et al. Front Cell Dev Biol. 2022 Sep 12;10:999511. doi: 10.3389/fcell.2022.999511. eCollection 2022. Front Cell Dev Biol. 2022. PMID: 36172287 Free PMC article. - d-Serine controls epidermal vesicle release via NMDA receptor, allowing tissue migration during the metamorphosis of the chordate Ciona.
Krasovec G, Hozumi A, Yoshida T, Obita T, Hamada M, Shiraishi A, Satake H, Horie T, Mori H, Sasakura Y. Krasovec G, et al. Sci Adv. 2022 Mar 11;8(10):eabn3264. doi: 10.1126/sciadv.abn3264. Epub 2022 Mar 11. Sci Adv. 2022. PMID: 35275721 Free PMC article. - Mesmerize is a dynamically adaptable user-friendly analysis platform for 2D and 3D calcium imaging data.
Kolar K, Dondorp D, Zwiggelaar JC, Høyer J, Chatzigeorgiou M. Kolar K, et al. Nat Commun. 2021 Nov 12;12(1):6569. doi: 10.1038/s41467-021-26550-y. Nat Commun. 2021. PMID: 34772921 Free PMC article. - Vasopressin Promoter Transgenic and Vasopressin Gene-Edited Ascidian, Ciona intestinalis Type A (Ciona robusta): Innervation, Gene Expression Profiles, and Phenotypes.
Kawada T, Shiraishi A, Matsubara S, Hozumi A, Horie T, Sasakura Y, Satake H. Kawada T, et al. Front Endocrinol (Lausanne). 2021 May 6;12:668564. doi: 10.3389/fendo.2021.668564. eCollection 2021. Front Endocrinol (Lausanne). 2021. PMID: 34025581 Free PMC article. - Differential Expression of a Classic Cadherin Directs Tissue-Level Contractile Asymmetry during Neural Tube Closure.
Hashimoto H, Munro E. Hashimoto H, et al. Dev Cell. 2019 Oct 21;51(2):158-172.e4. doi: 10.1016/j.devcel.2019.10.001. Dev Cell. 2019. PMID: 31639367 Free PMC article.
References
- Nat Genet. 2003 Nov;35(3):213-4 - PubMed
- Gene. 2003 Apr 10;308:11-20 - PubMed
- Proc Natl Acad Sci U S A. 2004 Jan 27;101(4):986-91 - PubMed
- Dev Growth Differ. 2007 Jun;49(5):373-82 - PubMed
- Nucleic Acids Res. 1991 Dec 11;19(23):6646 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous