Down-regulation of miR-27a might reverse multidrug resistance of esophageal squamous cell carcinoma - PubMed (original) (raw)

. 2010 Sep;55(9):2545-51.

doi: 10.1007/s10620-009-1051-6. Epub 2009 Dec 4.

Affiliations

• PMID: 19960259
• DOI: 10.1007/s10620-009-1051-6

Down-regulation of miR-27a might reverse multidrug resistance of esophageal squamous cell carcinoma

Hongwei Zhang et al. Dig Dis Sci. 2010 Sep.

Abstract

Background: So far, the miRNAs involved in multidrug resistance of esophageal cancer have not been reported.

Aims and methods: Here we have firstly investigated the roles of miR-27a in multidrug resistance of esophageal squamous cell carcinoma using MTT assay, flow cytometry assay, and reporter gene assay, etc.

Results: Down-regulation of miR-27a could confer sensitivity of both P-glycoprotein-related and P-glycoprotein-non-related drugs on esophageal cancer cells, and might promote ADR-induced apoptosis, accompanied by increased accumulation and decreased releasing amount of ADR. Down-regulation of miR-27a could significantly decrease the expression of P-glycoprotein, Bcl-2, and the transcription of the multidrug resistance gene 1, but up-regulate the expression of Bax.

Conclusions: MiR-27a might play important roles in multidrug resistance of esophageal cancer. The further study of the biological functions of miR-27a might be helpful for developing possible strategies to treat esophageal cancer.

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