Proteome organization in a genome-reduced bacterium - PubMed (original) (raw)
. 2009 Nov 27;326(5957):1235-40.
doi: 10.1126/science.1176343.
Vera van Noort, Matthew J Betts, Alejandra Leo-Macias, Claire Batisse, Michaela Rode, Takuji Yamada, Tobias Maier, Samuel Bader, Pedro Beltran-Alvarez, Daniel Castaño-Diez, Wei-Hua Chen, Damien Devos, Marc Güell, Tomas Norambuena, Ines Racke, Vladimir Rybin, Alexander Schmidt, Eva Yus, Ruedi Aebersold, Richard Herrmann, Bettina Böttcher, Achilleas S Frangakis, Robert B Russell, Luis Serrano, Peer Bork, Anne-Claude Gavin
Affiliations
- PMID: 19965468
- DOI: 10.1126/science.1176343
Proteome organization in a genome-reduced bacterium
Sebastian Kühner et al. Science. 2009.
Abstract
The genome of Mycoplasma pneumoniae is among the smallest found in self-replicating organisms. To study the basic principles of bacterial proteome organization, we used tandem affinity purification-mass spectrometry (TAP-MS) in a proteome-wide screen. The analysis revealed 62 homomultimeric and 116 heteromultimeric soluble protein complexes, of which the majority are novel. About a third of the heteromultimeric complexes show higher levels of proteome organization, including assembly into larger, multiprotein complex entities, suggesting sequential steps in biological processes, and extensive sharing of components, implying protein multifunctionality. Incorporation of structural models for 484 proteins, single-particle electron microscopy, and cellular electron tomograms provided supporting structural details for this proteome organization. The data set provides a blueprint of the minimal cellular machinery required for life.
Comment in
- Systems biology. Excavating the functional landscape of bacterial cells.
Ochman H, Raghavan R. Ochman H, et al. Science. 2009 Nov 27;326(5957):1200-1. doi: 10.1126/science.1183757. Science. 2009. PMID: 19965458 No abstract available.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous