The extracellular matrix in hepatic regeneration. Localization of collagen types I, III, IV, laminin, and fibronectin - PubMed (original) (raw)
Affiliations
- PMID: 1997730
The extracellular matrix in hepatic regeneration. Localization of collagen types I, III, IV, laminin, and fibronectin
A Martinez-Hernandez et al. Lab Invest. 1991 Feb.
Erratum in
- Lab Invest 1991 Aug;65(2):257
Abstract
After partial hepatectomy, the liver is capable of complete regeneration, restoring normal hepatic size, architecture, and function. To study the role of the extracellular matrix in regeneration, the temporal and spatial sequence of deposition of several of its components, including collagen types I, III, and IV, laminin, and fibronectin, in rat liver, after an 80% hepatectomy, was characterized by light microscopy immunohistochemistry. A minimum of five animals were studied for each date. In agreement with previous reports, subsequent to 80% hepatectomy, there was a brisk mitosis of hepatocytes. The mitotic activity was maximal at 48 hours, primarily in the periportal and centrilobular zones, and resulted in the formation of hepatocyte clusters and widening of the hepatic plates. Of the extracellular matrix components studied, laminin was the one demonstrating the most dramatic changes. By 24 hours, laminin appeared in the hepatic sinusoids reaching a maximum staining intensity at 48 hours. Intracellular laminin was prominent in numerous non-parenchymal cells, with many having the morphology, location, and desmin content characteristic of Ito cells. Laminin staining decreased in the sinusoids at 4 days; however, some intracellular staining of Ito cells was present even at 8 days after hepatectomy. At the completion of regeneration, there was no evidence of any substantial change in the ratio: extracellular matrix/cell mass. The results indicate that: (a) hepatocytes can divide without prior removal of the subsinusoidal extracellular matrix; (b) during regeneration, hepatocyte division precedes sinusoidal formation; (c) during hepatic regeneration, and in spite of the presence of laminin in Ito cells, no basement membranes are formed; (d) the prominent expression of laminin and its proposed functions in morphogenesis suggest a critical role for this matrix component in the formation and reorganization of the regenerating liver.
Similar articles
- Expression and potential role of the extracellular matrix in hepatic ontogenesis: a review.
Amenta PS, Harrison D. Amenta PS, et al. Microsc Res Tech. 1997 Nov 15;39(4):372-86. doi: 10.1002/(SICI)1097-0029(19971115)39:4<372::AID-JEMT7>3.0.CO;2-J. Microsc Res Tech. 1997. PMID: 9407547 Review. - The hepatic extracellular matrix. II. Electron immunohistochemical studies in rats with CCl4-induced cirrhosis.
Martinez-Hernandez A. Martinez-Hernandez A. Lab Invest. 1985 Aug;53(2):166-86. Lab Invest. 1985. PMID: 3894794 Review. - The hepatic extracellular matrix. I. Electron immunohistochemical studies in normal rat liver.
Martinez-Hernandez A. Martinez-Hernandez A. Lab Invest. 1984 Jul;51(1):57-74. Lab Invest. 1984. PMID: 6376944 - Cellular sources of matrix proteins in experimentally induced cholestatic rat liver.
Abdel-Aziz G, Rescan PY, Clement B, Lebeau G, Rissel M, Grimaud JA, Campion JP, Guillouzo A. Abdel-Aziz G, et al. J Pathol. 1991 Jun;164(2):167-74. doi: 10.1002/path.1711640211. J Pathol. 1991. PMID: 2072216
Cited by
- Production and activity of matrix metalloproteinases during liver fibrosis progression of chronic hepatitis C patients.
Martinez-Castillo M, Hernandez-Barragan A, Flores-Vasconcelos I, Galicia-Moreno M, Rosique-Oramas D, Perez-Hernandez JL, Higuera-De la Tijera F, Montalvo-Jave EE, Torre-Delgadillo A, Cordero-Perez P, Muñoz-Espinosa L, Kershenobich D, Gutierrez-Reyes G. Martinez-Castillo M, et al. World J Hepatol. 2021 Feb 27;13(2):218-232. doi: 10.4254/wjh.v13.i2.218. World J Hepatol. 2021. PMID: 33708351 Free PMC article. - Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells.
Wang Y, Alhaque S, Cameron K, Meseguer-Ripolles J, Lucendo-Villarin B, Rashidi H, Hay DC. Wang Y, et al. J Vis Exp. 2017 Mar 2;(121):55355. doi: 10.3791/55355. J Vis Exp. 2017. PMID: 28287600 Free PMC article. - Laminin matrix promotes hepatogenic terminal differentiation of human bone marrow mesenchymal stem cells.
Khalaj Z, Lotfi AS, Kabir-Salmani M. Khalaj Z, et al. Iran J Basic Med Sci. 2016 Jan;19(1):34-42. Iran J Basic Med Sci. 2016. PMID: 27096062 Free PMC article. - Biomaterials for liver tissue engineering.
Jain E, Damania A, Kumar A. Jain E, et al. Hepatol Int. 2014 Apr;8(2):185-97. doi: 10.1007/s12072-013-9503-7. Epub 2013 Dec 27. Hepatol Int. 2014. PMID: 26202501 - Amphiregulin activates human hepatic stellate cells and is upregulated in non alcoholic steatohepatitis.
McKee C, Sigala B, Soeda J, Mouralidarane A, Morgan M, Mazzoccoli G, Rappa F, Cappello F, Cabibi D, Pazienza V, Selden C, Roskams T, Vinciguerra M, Oben JA. McKee C, et al. Sci Rep. 2015 Mar 6;5:8812. doi: 10.1038/srep08812. Sci Rep. 2015. PMID: 25744849 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources