Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells - PubMed (original) (raw)
Effect of proinflammatory cytokines on the expression and regulation of human beta-defensin 2 in human dental pulp cells
Young-Suk Kim et al. J Endod. 2010 Jan.
Abstract
Introduction: Although the expression of human beta-defensin-2 (hBD-2) in odontoblasts from human dental pulp (HDP) has been reported, the production of hBD-2 and its regulation remains poorly understood. The aim of this study was to investigate the effect of cytokines on the induction of hBD-2 and its signaling mechanisms in HDP cells.
Methods: After stimulation with tumor necrosis factor alpha (TNF-alpha) and interleukin 1 alpha (IL-1 alpha), reverse-transcriptase polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay experiments were performed to evaluate the effects of these cytokines on the production of hBD-2.
Results: TNF-alpha and IL-1 alpha synergistically increased hBD-2 messenger RNA levels, protein expression, and activity. The up-regulation of hBD-2 by cytokines was attenuated by pretreatment with inhibitors of PKC, JNK, p38, ERK MAPK, nuclear factor-kappaB, and adenosine monophosphate-activated protein kinase (AMPK).
Conclusion: These results suggest that TNF-alpha and IL-1 alpha up-regulate HBD-2 expression in HDP cells through the PKC, JNK MAPK, p38, ERK, NF-kappaB, and AMPK pathways. Thus, the induction of hBD-2 by proinflammatory cytokines might up-regulate the pulpal host immune defense system.
Similar articles
- Up-regulation of human beta-defensin 2 by interleukin-1beta in A549 cells: involvement of PI3K, PKC, p38 MAPK, JNK, and NF-kappaB.
Jang BC, Lim KJ, Paik JH, Kwon YK, Shin SW, Kim SC, Jung TY, Kwon TK, Cho JW, Baek WK, Kim SP, Suh MH, Suh SI. Jang BC, et al. Biochem Biophys Res Commun. 2004 Jul 30;320(3):1026-33. doi: 10.1016/j.bbrc.2004.06.049. Biochem Biophys Res Commun. 2004. PMID: 15240151 - Dexamethasone suppresses interleukin-1beta-induced human beta-defensin 2 mRNA expression: involvement of p38 MAPK, JNK, MKP-1, and NF-kappaB transcriptional factor in A549 cells.
Jang BC, Lim KJ, Suh MH, Park JG, Suh SI. Jang BC, et al. FEMS Immunol Med Microbiol. 2007 Oct;51(1):171-84. doi: 10.1111/j.1574-695X.2007.00293.x. Epub 2007 Jul 23. FEMS Immunol Med Microbiol. 2007. PMID: 17645739 - Inflammation and regeneration in the dentin-pulp complex: a double-edged sword.
Cooper PR, Holder MJ, Smith AJ. Cooper PR, et al. J Endod. 2014 Apr;40(4 Suppl):S46-51. doi: 10.1016/j.joen.2014.01.021. J Endod. 2014. PMID: 24698693 Review. - [Expression and regulation of beta-defensin 2 in respiratory system].
Sun BB, Wen FQ. Sun BB, et al. Zhonghua Jie He He Hu Xi Za Zhi. 2008 Sep;31(9):694-6. Zhonghua Jie He He Hu Xi Za Zhi. 2008. PMID: 19080577 Review. Chinese. No abstract available.
Cited by
- Sensitivity of caries pathogens to antimicrobial peptides related to caries risk.
Goeke JE, Kist S, Schubert S, Hickel R, Huth KC, Kollmuss M. Goeke JE, et al. Clin Oral Investig. 2018 Sep;22(7):2519-2525. doi: 10.1007/s00784-018-2348-7. Epub 2018 Jan 25. Clin Oral Investig. 2018. PMID: 29372443 - Local Defense Factors in Cleft-Affected Palate in Children before and during Milk Dentition Age: A Pilot Study.
Ozola L, Pilmane M. Ozola L, et al. J Pers Med. 2023 Dec 25;14(1):27. doi: 10.3390/jpm14010027. J Pers Med. 2023. PMID: 38248728 Free PMC article. - Therapeutic Potential of Synthetic Human _β_-Defensin 1 Short Motif Pep-B on Lipopolysaccharide-Stimulated Human Dental Pulp Stem Cells.
Shi J, Hu Z, Zhou Y, Zuo M, Wu H, Jin W, Xie Z. Shi J, et al. Mediators Inflamm. 2022 Jan 24;2022:6141967. doi: 10.1155/2022/6141967. eCollection 2022. Mediators Inflamm. 2022. PMID: 35110972 Free PMC article. - Activation and Biological Properties of Human β Defensin 4 in Stem Cells Derived From Human Exfoliated Deciduous Teeth.
Zhai Y, Wang Y, Rao N, Li J, Li X, Fang T, Zhao Y, Ge L. Zhai Y, et al. Front Physiol. 2019 Oct 22;10:1304. doi: 10.3389/fphys.2019.01304. eCollection 2019. Front Physiol. 2019. PMID: 31695620 Free PMC article. - Human β-Defensins in Diagnosis of Head and Neck Cancers.
Kompuinen J, Keskin M, Yilmaz D, Gürsoy M, Gürsoy UK. Kompuinen J, et al. Cells. 2023 Mar 7;12(6):830. doi: 10.3390/cells12060830. Cells. 2023. PMID: 36980171 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous