Comparison of the Abbott RealTime CT new formulation assay with two other commercial assays for detection of wild-type and new variant strains of Chlamydia trachomatis - PubMed (original) (raw)

Comparative Study

Comparison of the Abbott RealTime CT new formulation assay with two other commercial assays for detection of wild-type and new variant strains of Chlamydia trachomatis

Jens Kjølseth Møller et al. J Clin Microbiol. 2010 Feb.

Abstract

In an analytical-method comparison study of clinical samples, the Abbott RealTime CT new formulation assay (m2000 real-time PCR), consisting of a duplex PCR targeting different parts of the cryptic plasmid in Chlamydia trachomatis, was compared both with version 2 of the Roche Cobas TaqMan CT assay, comprising a duplex PCR for a target in the cryptic plasmid and the omp1 gene, and with the Gen-Probe Aptima Combo 2 assay (AC2) targeting the C. trachomatis 23S rRNA molecule. First-catch urine samples from Sweden were tested in Malmö, Sweden, for C. trachomatis with the m2000 real-time PCR assay and with an in-house PCR for the new variant C. trachomatis strain with a deletion in the cryptic plasmid. Aliquots of the urine samples were sent to Aarhus, Denmark, where they were further examined with the TaqMan CT and AC2 assays. A positive prevalence of 9.1% (148/1,632 urine samples examined) was detected according to the combined reference standard. The sensitivities and specificities of the three assays were as follows: for the Abbott m2000 assay, 95.3% (141/148) and 99.9% (1,483/1,485), respectively; for the Roche TaqMan assay, 82.4% (122/148) and 100.0% (1,485/1,485); and for the Gen-Probe AC2 assay, 99.3% (147/148) and 99.9% (1,484/1,485). The plasmid mutant strain was detected in 24% (36/148) of the C. trachomatis-positive samples. There is a difference in sensitivity between the new formulations of the Abbott and the Roche assays, but both assays detected the wild-type and new variant C. trachomatis strains equally well.

PubMed Disclaimer

Similar articles

Cited by

References

    1. Hadad, R., H. Fredlund, and M. Unemo. 2009. Evaluation of the new COBAS TaqMan CT Test v2.0 and the impact on the proportion of the new variant of Chlamydia trachomatis (nvCT) by introduction of diagnostics detecting nvCT (LightMix 480HT PCR) in Orebro county, Sweden. Sex. Transm. Infect. 85:190-193. - PubMed
    1. Møller, J. K., L. N. Pedersen, and K. Persson. 2008. Comparison of Gen-Probe transcription-mediated amplification, Abbott PCR, and Roche PCR assays for detection of wild-type and mutant plasmid strains of Chlamydia trachomatis in Sweden. J. Clin. Microbiol. 46:3892-3895. - PMC - PubMed
    1. Ripa, T., and P. Nilsson. 2006. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill. 11:E061109.2. - PubMed
    1. Ripa, T., and P. A. Nilsson. 2007. A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex. Transm. Dis. 34:255-256. - PubMed
    1. Söderblom, T., A. Blaxhult, H. Fredlund, and B. Herrmann. 2006. Impact of a genetic variant of Chlamydia trachomatis on national detection rates in Sweden. Euro Surveill. 11:E061207.1. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources