Mechanism of action of an acidic transcriptional activator in vitro - PubMed (original) (raw)
Mechanism of action of an acidic transcriptional activator in vitro
Y S Lin et al. Cell. 1991.
Abstract
Transcription of a eukaryotic structural gene by RNA polymerase II requires the ordered assembly of general transcription factors on the promoter to form a pre-initiation complex. Here we analyze affinity-purified complexes at various stages of assembly to determine the mechanism of action of an acidic transcriptional activator. We show that the activator can function in the absence of ATP and stimulates transcription by increasing the number of functional preinitiation complexes. The activator effects this increase by recruiting the general transcription factor TFIIB to the promoter. Using protein affinity chromatography we demonstrate a specific interaction between an acidic activating region and TFIIB. Based on these combined results, we propose that TFIIB is a direct target of an acidic activator.
Similar articles
- Eukaryotic activators function during multiple steps of preinitiation complex assembly.
Choy B, Green MR. Choy B, et al. Nature. 1993 Dec 9;366(6455):531-6. doi: 10.1038/366531a0. Nature. 1993. PMID: 8255291 - Proline-rich activator CTF1 targets the TFIIB assembly step during transcriptional activation.
Kim TK, Roeder RG. Kim TK, et al. Proc Natl Acad Sci U S A. 1994 May 10;91(10):4170-4. doi: 10.1073/pnas.91.10.4170. Proc Natl Acad Sci U S A. 1994. PMID: 8183887 Free PMC article. - Recruitment of TBP or TFIIB to a promoter proximal position leads to stimulation of RNA polymerase II transcription without activator proteins both in vivo and in vitro.
Huh JR, Park JM, Kim M, Carlson BA, Hatfield DL, Lee BJ. Huh JR, et al. Biochem Biophys Res Commun. 1999 Mar 5;256(1):45-51. doi: 10.1006/bbrc.1999.0280. Biochem Biophys Res Commun. 1999. PMID: 10066420 - How eukaryotic transcription activators increase assembly of preinitiation complexes.
Choy B, Roberts SG, Griffin LA, Green MR. Choy B, et al. Cold Spring Harb Symp Quant Biol. 1993;58:199-203. doi: 10.1101/sqb.1993.058.01.024. Cold Spring Harb Symp Quant Biol. 1993. PMID: 7956030 Review. No abstract available. - TFIIB and the regulation of transcription by RNA polymerase II.
Deng W, Roberts SG. Deng W, et al. Chromosoma. 2007 Oct;116(5):417-29. doi: 10.1007/s00412-007-0113-9. Epub 2007 Jun 26. Chromosoma. 2007. PMID: 17593382 Review.
Cited by
- Discrete promoter elements affect specific properties of RNA polymerase II transcription complexes.
Steinke JW, Kopytek SJ, Peterson DO. Steinke JW, et al. Nucleic Acids Res. 2000 Jul 15;28(14):2726-35. doi: 10.1093/nar/28.14.2726. Nucleic Acids Res. 2000. PMID: 10908329 Free PMC article. - Considerations of transcriptional control mechanisms: do TFIID-core promoter complexes recapitulate nucleosome-like functions?
Hoffmann A, Oelgeschläger T, Roeder RG. Hoffmann A, et al. Proc Natl Acad Sci U S A. 1997 Aug 19;94(17):8928-35. doi: 10.1073/pnas.94.17.8928. Proc Natl Acad Sci U S A. 1997. PMID: 9256411 Free PMC article. Review. - The basic helix-loop-helix-zipper domain of TFE3 mediates enhancer-promoter interaction.
Artandi SE, Cooper C, Shrivastava A, Calame K. Artandi SE, et al. Mol Cell Biol. 1994 Dec;14(12):7704-16. doi: 10.1128/mcb.14.12.7704-7716.1994. Mol Cell Biol. 1994. PMID: 7969114 Free PMC article. - Oestrogen receptor facilitates the formation of preinitiation complex assembly: involvement of the general transcription factor TFIIB.
Sabbah M, Kang KI, Tora L, Redeuilh G. Sabbah M, et al. Biochem J. 1998 Dec 15;336 ( Pt 3)(Pt 3):639-46. doi: 10.1042/bj3360639. Biochem J. 1998. PMID: 9841876 Free PMC article. - Protein and DNA elements involved in transactivation of the promoter of the bovine herpesvirus (BHV) 1 IE-1 transcription unit by the BHV alpha gene trans-inducing factor.
Misra V, Bratanich AC, Carpenter D, O'Hare P. Misra V, et al. J Virol. 1994 Aug;68(8):4898-909. doi: 10.1128/JVI.68.8.4898-4909.1994. J Virol. 1994. PMID: 8035488 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical