Food reservoir for Escherichia coli causing urinary tract infections - PubMed (original) (raw)

Food reservoir for Escherichia coli causing urinary tract infections

Caroline Vincent et al. Emerg Infect Dis. 2010 Jan.

Abstract

Closely related strains of Escherichia coli have been shown to cause extraintestinal infections in unrelated persons. This study tests whether a food reservoir may exist for these E. coli. Isolates from 3 sources over the same time period (2005-2007) and geographic area were compared. The sources comprised prospectively collected E. coli isolates from women with urinary tract infection (UTI) (n = 353); retail meat (n = 417); and restaurant/ready-to-eat foods (n = 74). E. coli were evaluated for antimicrobial drug susceptibility and O:H serotype and compared by using 4 different genotyping methods. We identified 17 clonal groups that contained E. coli isolates (n = 72) from >1 source. E. coli from retail chicken (O25:H4-ST131 and O114:H4-ST117) and honeydew melon (O2:H7-ST95) were indistinguishable from or closely related to E. coli from human UTIs. This study provides strong support for the role of food reservoirs or foodborne transmission in the dissemination of E. coli causing common community-acquired UTIs.

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Figures

Figure 1

Figure 1

Pulsed-field gel electrophoresis patterns for Escherichia coli O2:H7-ST95 and E. coli O25:H4-ST131. A) _Xba_I; B) _Not_I. Lane 1 is the positive control E. coli O11:H18-ST69 (SEQ102); lane 2 is an E. coli O2:H7-ST95 isolate from a restaurant sample of honeydew melon (68616.01); lanes 3–10 are isolates from human urinary tract infection cases (UTIs; lane 3, MSHS 100; lane 4, MSHS 186; lane 5, MSHS 811; lane 6, MSHS 1229; lane 7, MSHS 95; lane 8, MSHS 1062; lane 9, MSHS 782; lane 10, MSHS 819); lane 11 is an E. coli O25:H4-ST131 isolate from a retail chicken sample (EC01DT06-1737-01); and lanes 12 and 13 are E. coli isolates from human UTIs (lane 12, MSHS 161; lane 13, MSHS 1134A). Outer lanes are pulsed-field molecular weight markers.

Figure 2

Figure 2

_Xba_I and _Not_I pulsed-field gel electrophoresis patterns for Escherichia coli O114:H4-ST117 (lanes 2 and 3). Lane 1 is the positive control E. coli O11:H18-ST69 (SEQ102), lane 2 is an E. coli O25:H4-ST131 isolate from a retail chicken sample (EC01DT06-1737-01), and lane 3 is an E. coli isolate from a human urinary tract infection case (MSHS 1014A). Outer and center lanes are pulsed-field molecular weight markers.

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