Extractable collagenase and carcinogenesis of the mouse skin - PubMed (original) (raw)
Extractable collagenase and carcinogenesis of the mouse skin
G Wirl. Connect Tissue Res. 1977.
Abstract
Collagenolytic activity has been demonstrated in the early phase of chemical carcinogenesis of mouse skin following 3-methylcholanthrene application dropwise in acetone or painted on the skin in benzene. In addition very high levels of collagenase could be detected in mouse skin papillomas and carcinomas. In all the tissues investigated, collagenase activity was extracted from the 6000 X g sediment of tissue homogenates with 5 M urea in 50 mM Tris-HCl buffer, pH 7.5. After dialyzing the extract, the enzyme was precipitated with ammonium sulfate and the activity determined against 14C-collagen substrate in solution. This procedure was found suitable for the detection and estimation of collagenase activity in skin tissues with high turnover of collagen and thus offers an attractive alternative to tissue culture methods.
Similar articles
- Reduction in IkappaB kinase alpha expression promotes the development of skin papillomas and carcinomas.
Park E, Zhu F, Liu B, Xia X, Shen J, Bustos T, Fischer SM, Hu Y. Park E, et al. Cancer Res. 2007 Oct 1;67(19):9158-68. doi: 10.1158/0008-5472.CAN-07-0590. Cancer Res. 2007. PMID: 17909021 - Extraction of collagenase from the 6000 times g sediment of uterine and skin tissues of mice. A comparative study.
Wirl G. Wirl G. Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1289-95. doi: 10.1515/bchm2.1975.356.2.1289. Hoppe Seylers Z Physiol Chem. 1975. PMID: 170181 - [Collagenase activity in an animal and a human carcinoma (author's transl)].
Wirl G. Wirl G. Wien Klin Wochenschr. 1977 Nov 25;89(22):766-8. Wien Klin Wochenschr. 1977. PMID: 201108 German. - Development of murine skin neoplasia studied with enzymes used as cell markers.
Reddy AL, Fialkow PJ. Reddy AL, et al. Carcinog Compr Surv. 1989;11:213-26. Carcinog Compr Surv. 1989. PMID: 2493335 Review. No abstract available.
Cited by
- The role of the macrophage in collagen resorption during chronic inflammation. A new look at an old hypothesis.
Deporter DA. Deporter DA. Agents Actions. 1979 Jun;9(2):168-71. doi: 10.1007/BF02024729. Agents Actions. 1979. PMID: 224681 Review. No abstract available. - A novel leupeptin-sensitive serine endopeptidase present in normal and malignant rat mammary tissues.
Eto I, Bandy MD. Eto I, et al. Mol Cell Biochem. 1990 Apr 18;94(1):19-36. doi: 10.1007/BF00223559. Mol Cell Biochem. 1990. PMID: 2166230 - Collagenolytic activity in experimental intestinal anastomoses. Differences between small and large bowel and evidence for the presence of collagenase.
van der Stappen JW, Hendriks T, de Boer HH, de Man BM, de Pont JJ. van der Stappen JW, et al. Int J Colorectal Dis. 1992 Jun;7(2):95-101. doi: 10.1007/BF00341294. Int J Colorectal Dis. 1992. PMID: 1319443 - Effect of lupeol and lupeol linoleate on lysosomal enzymes and collagen in adjuvant-induced arthritis in rats.
Geetha T, Varalakshmi P. Geetha T, et al. Mol Cell Biochem. 1999 Nov;201(1-2):83-7. doi: 10.1023/a:1007056300503. Mol Cell Biochem. 1999. PMID: 10630626 - Proteolytic and metastatic activities of clones derived from a methylcholanthrene-induced murine fibrosarcoma.
McLaughlin ME, Liener IE, Wang N. McLaughlin ME, et al. Clin Exp Metastasis. 1983 Oct-Dec;1(4):359-71. doi: 10.1007/BF00121198. Clin Exp Metastasis. 1983. PMID: 6100805
MeSH terms
Substances
LinkOut - more resources
Full Text Sources