A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family - PubMed (original) (raw)
Comparative Study
doi: 10.1101/gad.5.4.629.
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- PMID: 2010088
- DOI: 10.1101/gad.5.4.629
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Comparative Study
A cold-sensitive mRNA splicing mutant is a member of the RNA helicase gene family
E J Strauss et al. Genes Dev. 1991 Apr.
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Abstract
We have isolated a cold-sensitive mutant of Saccharomyces cerevisiae in which the first step of mRNA splicing is inhibited. The growth and splicing defects are recessive and cosegregate, thus defining a single essential gene (PRP28). The wild-type PRP28 gene was cloned, and sequence analysis reveals extensive homology to a family of proteins that are thought to function as ATP-dependent RNA helicases. The cold sensitivity is caused by a glycine-to-glutamic acid change in a conserved sequence motif. Interestingly, double mutants containing conditional alleles of PRP28 and PRP24, which encodes a U6 snRNA-binding protein, are inviable. In addition, a suppressor of prp28-1 is a mutant allele of PRP8, which encodes a U5 protein, thus linking PRP28 with U5. These data are consistent with a scenario in which PRP28 acts to unwind the U4/U6 base-pairing interaction in the U4/U6/U5 snRNP, facilitating the first covalent step of splicing.
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