Current limitations in native mass spectrometry based structural biology - PubMed (original) (raw)
Current limitations in native mass spectrometry based structural biology
Esther van Duijn. J Am Soc Mass Spectrom. 2010 Jun.
Free article
Abstract
Nowadays, mass spectrometry plays an important role in structural biology. At one end it can be used to investigate intact protein complexes, providing details about the complex composition, topology, stability, and dynamics, whereas at the other end the protein's identity and possible modifications can be visualized using proteomics approaches. Combining all this information allows the generation of detailed models for functional biological assemblies. Here, a perspective on the application of native mass spectrometry in structural biology is presented. The potential of this technique and some important current limitations are discussed. This includes issues regarding the quality/homogeneity of the sample, the dissociation efficiency of protein complexes during tandem mass spectrometric analysis, and some boundaries of ion mobility mass spectrometry.
Copyright 2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.
Similar articles
- Surface induced dissociation: dissecting noncovalent protein complexes in the gas phase.
Zhou M, Wysocki VH. Zhou M, et al. Acc Chem Res. 2014 Apr 15;47(4):1010-8. doi: 10.1021/ar400223t. Epub 2014 Feb 13. Acc Chem Res. 2014. PMID: 24524650 - Native mass spectrometry as a tool in structural biology.
Lorenzen K, van Duijn E. Lorenzen K, et al. Curr Protoc Protein Sci. 2010 Nov;Chapter 17:Unit17.12. doi: 10.1002/0471140864.ps1712s62. Curr Protoc Protein Sci. 2010. PMID: 21104986 - Improving the performance of a quadrupole time-of-flight instrument for macromolecular mass spectrometry.
van den Heuvel RH, van Duijn E, Mazon H, Synowsky SA, Lorenzen K, Versluis C, Brouns SJ, Langridge D, van der Oost J, Hoyes J, Heck AJ. van den Heuvel RH, et al. Anal Chem. 2006 Nov 1;78(21):7473-83. doi: 10.1021/ac061039a. Anal Chem. 2006. PMID: 17073415 - Glycoproteomics based on tandem mass spectrometry of glycopeptides.
Wuhrer M, Catalina MI, Deelder AM, Hokke CH. Wuhrer M, et al. J Chromatogr B Analyt Technol Biomed Life Sci. 2007 Apr 15;849(1-2):115-28. doi: 10.1016/j.jchromb.2006.09.041. Epub 2006 Oct 17. J Chromatogr B Analyt Technol Biomed Life Sci. 2007. PMID: 17049937 Review. - Ion mobility mass spectrometry of proteins and protein assemblies.
Uetrecht C, Rose RJ, van Duijn E, Lorenzen K, Heck AJ. Uetrecht C, et al. Chem Soc Rev. 2010 May;39(5):1633-55. doi: 10.1039/b914002f. Epub 2009 Nov 25. Chem Soc Rev. 2010. PMID: 20419213 Review.
Cited by
- Simultaneous Native Mass Spectrometry Analysis of Single and Double Mutants To Probe Lipid Binding to Membrane Proteins.
Jayasekera HS, Mohona FA, Ewbank M, Marty MT. Jayasekera HS, et al. Anal Chem. 2024 Jun 25;96(25):10426-10433. doi: 10.1021/acs.analchem.4c01704. Epub 2024 Jun 10. Anal Chem. 2024. PMID: 38859611 - Mechanism of Viral DNA Packaging in Phage T4 Using Single-Molecule Fluorescence Approaches.
Dasgupta S, Thomas JA, Ray K. Dasgupta S, et al. Viruses. 2024 Jan 26;16(2):192. doi: 10.3390/v16020192. Viruses. 2024. PMID: 38399968 Free PMC article. Review. - SIMULTANEOUS NATIVE MASS SPECTROMETRY ANALYSIS OF SINGLE AND DOUBLE MUTANTS TO PROBE LIPID BINDING TO MEMBRANE PROTEINS.
Jayasekera HS, Mohona FA, Ewbank M, Marty MT. Jayasekera HS, et al. bioRxiv [Preprint]. 2023 Dec 12:2023.09.19.558516. doi: 10.1101/2023.09.19.558516. bioRxiv. 2023. PMID: 37781586 Free PMC article. Updated. Preprint. - Bacteriophage T4 Head: Structure, Assembly, and Genome Packaging.
Rao VB, Fokine A, Fang Q, Shao Q. Rao VB, et al. Viruses. 2023 Feb 14;15(2):527. doi: 10.3390/v15020527. Viruses. 2023. PMID: 36851741 Free PMC article. Review. - Characterization of the T4 gp32-ssDNA complex by native, cross-linking, and ultraviolet photodissociation mass spectrometry.
Blevins MS, Walker JN, Schaub JM, Finkelstein IJ, Brodbelt JS. Blevins MS, et al. Chem Sci. 2021 Sep 23;12(41):13764-13776. doi: 10.1039/d1sc02861h. eCollection 2021 Oct 27. Chem Sci. 2021. PMID: 34760161 Free PMC article.
References
- J Biol Chem. 2008 Oct 17;283(42):28513-7 - PubMed
- Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18139-44 - PubMed
- Curr Opin Chem Biol. 2004 Oct;8(5):519-26 - PubMed
- Chemistry. 2005 Jan 21;11(3):960-8 - PubMed
- Curr Protoc Protein Sci. 2010 Nov;Chapter 17:Unit17.12 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources