Cellular internalization of exosomes occurs through phagocytosis - PubMed (original) (raw)
Cellular internalization of exosomes occurs through phagocytosis
Du Feng et al. Traffic. 2010 May.
Free article
Abstract
Exosomes play important roles in many physiological and pathological processes. However, the exosome-cell interaction mode and the intracellular trafficking pathway of exosomes in their recipient cells remain unclear. Here, we report that exosomes derived from K562 or MT4 cells are internalized more efficiently by phagocytes than by non-phagocytic cells. Most exosomes were observed attached to the plasma membrane of non-phagocytic cells, while in phagocytic cells these exosomes were found to enter via phagocytosis. Specifically, they moved to phagosomes together with phagocytic polystyrene carboxylate-modified latex beads (biospheres) and were further sorted into phagolysosomes. Moreover, exosome internalization was dependent on the actin cytoskeleton and phosphatidylinositol 3-kinase, and could be inhibited by the knockdown of dynamin2 or overexpression of a dominant-negative form of dynamin2. Further, antibody pretreatment assays demonstrated that tim4 but not tim1 was involved in exosomes uptake. We also found that exosomes did not enter the internalization pathway involving caveolae, macropinocytosis and clathrin-coated vesicles. Our observation that the cellular uptake of exosomes occurs through phagocytosis has important implications for exosome-cell interactions and the exosome intracellular trafficking pathway.
Similar articles
- Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy.
Tian T, Wang Y, Wang H, Zhu Z, Xiao Z. Tian T, et al. J Cell Biochem. 2010 Oct 1;111(2):488-96. doi: 10.1002/jcb.22733. J Cell Biochem. 2010. PMID: 20533300 - Activation-induced internalization differs for the tetraspanins CD9 and Tspan8: Impact on tumor cell motility.
Rana S, Claas C, Kretz CC, Nazarenko I, Zoeller M. Rana S, et al. Int J Biochem Cell Biol. 2011 Jan;43(1):106-19. doi: 10.1016/j.biocel.2010.10.002. Epub 2010 Oct 16. Int J Biochem Cell Biol. 2011. PMID: 20937409 - Proteomic profiling of exosomes: current perspectives.
Simpson RJ, Jensen SS, Lim JW. Simpson RJ, et al. Proteomics. 2008 Oct;8(19):4083-99. doi: 10.1002/pmic.200800109. Proteomics. 2008. PMID: 18780348 Review. - Exosomes--vesicular carriers for intercellular communication.
Simons M, Raposo G. Simons M, et al. Curr Opin Cell Biol. 2009 Aug;21(4):575-81. doi: 10.1016/j.ceb.2009.03.007. Epub 2009 May 11. Curr Opin Cell Biol. 2009. PMID: 19442504 Review.
Cited by
- Exosome-like Nanoparticles, High in Trans-δ-Viniferin Derivatives, Produced from Grape Cell Cultures: Preparation, Characterization, and Anticancer Properties.
Shkryl Y, Tsydeneshieva Z, Menchinskaya E, Rusapetova T, Grishchenko O, Mironova A, Bulgakov D, Gorpenchenko T, Kazarin V, Tchernoded G, Bulgakov V, Aminin D, Yugay Y. Shkryl Y, et al. Biomedicines. 2024 Sep 20;12(9):2142. doi: 10.3390/biomedicines12092142. Biomedicines. 2024. PMID: 39335655 Free PMC article. - Exosome Mediated Cell-Cell Crosstalk in Tissue Injury and Repair.
Yadav A, Nandy A, Sharma A, Ghatak S. Yadav A, et al. Results Probl Cell Differ. 2024;73:249-297. doi: 10.1007/978-3-031-62036-2_12. Results Probl Cell Differ. 2024. PMID: 39242383 Review. - Extracellular Vesicles as Drug Delivery System for Cancer Therapy.
Wang J, Yin B, Lian J, Wang X. Wang J, et al. Pharmaceutics. 2024 Aug 1;16(8):1029. doi: 10.3390/pharmaceutics16081029. Pharmaceutics. 2024. PMID: 39204374 Free PMC article. Review. - Role of Extracellular Vesicles in the Progression of Brain Tumors.
Schiera G, Di Liegro CM, Vento F, Di Liegro I. Schiera G, et al. Biology (Basel). 2024 Aug 2;13(8):586. doi: 10.3390/biology13080586. Biology (Basel). 2024. PMID: 39194524 Free PMC article. Review. - Youthful small extracellular vesicles restore the function and reparative capacity of inflammatory-impaired periodontal ligament stem cells via delivering protein biglycan for bone regeneration.
Yang J, Su J, Sun Z, Song Y, Zhang Y, Zhang Z, Wei J, Shi X, Jiang N, Ge X. Yang J, et al. J Nanobiotechnology. 2024 Aug 24;22(1):508. doi: 10.1186/s12951-024-02752-6. J Nanobiotechnology. 2024. PMID: 39182069 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources