Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury - PubMed (original) (raw)

doi: 10.1016/j.scr.2009.12.003. Epub 2010 Jan 4.

Fatih Arslan, May May Lee, Newman Siu Kwan Sze, Andre Choo, Tian Sheng Chen, Manuel Salto-Tellez, Leo Timmers, Chuen Neng Lee, Reida Menshawe El Oakley, Gerard Pasterkamp, Dominique P V de Kleijn, Sai Kiang Lim

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• PMID: 20138817
• DOI: 10.1016/j.scr.2009.12.003

Free article

Exosome secreted by MSC reduces myocardial ischemia/reperfusion injury

Ruenn Chai Lai et al. Stem Cell Res. 2010 May.

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Abstract

Human ESC-derived mesenchymal stem cell (MSC)-conditioned medium (CM) was previously shown to mediate cardioprotection during myocardial ischemia/reperfusion injury through large complexes of 50-100 nm. Here we show that these MSCs secreted 50- to 100-nm particles. These particles could be visualized by electron microscopy and were shown to be phospholipid vesicles consisting of cholesterol, sphingomyelin, and phosphatidylcholine. They contained coimmunoprecipitating exosome-associated proteins, e.g., CD81, CD9, and Alix. These particles were purified as a homogeneous population of particles with a hydrodynamic radius of 55-65 nm by size-exclusion fractionation on a HPLC. Together these observations indicated that these particles are exosomes. These purified exosomes reduced infarct size in a mouse model of myocardial ischemia/reperfusion injury. Therefore, MSC mediated its cardioprotective paracrine effect by secreting exosomes. This novel role of exosomes highlights a new perspective into intercellular mediation of tissue injury and repair, and engenders novel approaches to the development of biologics for tissue repair.

Copyright 2009 Elsevier B.V. All rights reserved.

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