Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII - PubMed (original) (raw)

. 1991 Apr 15;266(11):6888-93.

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Identification of the site of autophosphorylation of the bacterial protein kinase/phosphatase NRII

A J Ninfa et al. J Biol Chem. 1991.

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Abstract

Previous studies have established that the Escherichia coli protein kinase/phosphatase nitrogen regulator II (NRII also known as NtrB) becomes autophosphorylated on a histidine residue when incubated with ATP. We show that the major site at which NRII was autophosphorylated was contained within a peptide consisting of amino acid residues 136-142 of NRII, and thus probably corresponds to His-139. A minor site of phosphorylation, accounting for about 2% of the phosphate in NRII-P, was found in a peptide that corresponds to residues 158-169.

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