Identification of foodborne pathogens by nucleic acid hybridization - PubMed (original) (raw)
Review
Identification of foodborne pathogens by nucleic acid hybridization
W E Hill et al. Int J Food Microbiol. 1991 Jan.
Abstract
Nucleic acid hybridization methods have been developed and used to identify microorganisms in foods. Tests performed on mixed cultures save the time required to establish pure cultures. Enterotoxigenic or invasive strains of foodborne bacterial pathogens are detected with probes that identify genes responsible for virulence. Hybridization tests signal the presence or absence of a particular strain or an entire genus and are especially well suited for screening foods for specific pathogens. With the colony hybridization assay format, foodborne bacteria harboring a specific gene can be enumerated. However, hybridization tests require the presence of 10(5) to 10(6) cells to yield a positive result, thereby limiting sensitivity and necessitating a time-consuming growth step. In vitro DNA amplification techniques increase the amount of DNA segments 10(5)-10(6)-fold in 2 to 3 h, thus enhancing test sensitivity.
Similar articles
- Detection of foodborne pathogens using DNA probes and a dipstick format.
Groody EP. Groody EP. Methods Mol Biol. 1994;28:217-24. doi: 10.1385/0-89603-254-x:217. Methods Mol Biol. 1994. PMID: 8118511 No abstract available. - The use of plasmid profiles and nucleic acid probes in epidemiologic investigations of foodborne, diarrheal diseases.
Wachsmuth IK, Kiehlbauch JA, Bopp CA, Cameron DN, Strockbine NA, Wells JG, Blake PA. Wachsmuth IK, et al. Int J Food Microbiol. 1991 Jan;12(1):77-89. doi: 10.1016/0168-1605(91)90049-u. Int J Food Microbiol. 1991. PMID: 2018708 - [Molecular-genetic analysis of foodborne pathogens].
Efimochkina NR. Efimochkina NR. Vopr Pitan. 2007;76(2):4-15. Vopr Pitan. 2007. PMID: 17561648 Review. Russian. - Evaluation and interpretation of data obtained with immunoassays and DNA-DNA hybridization techniques.
Notermans S, Wernars K. Notermans S, et al. Int J Food Microbiol. 1990 Aug;11(1):35-49. doi: 10.1016/0168-1605(90)90038-7. Int J Food Microbiol. 1990. PMID: 2223520 Review. - Applications of nucleic acid probes in veterinary infectious diseases.
Paul PS. Paul PS. Vet Microbiol. 1990 Sep;24(3-4):409-17. doi: 10.1016/0378-1135(90)90187-z. Vet Microbiol. 1990. PMID: 2124400 Free PMC article. Review.
Cited by
- Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR.
Khan AA, Cerniglia CE. Khan AA, et al. Appl Environ Microbiol. 1994 Oct;60(10):3739-45. doi: 10.1128/aem.60.10.3739-3745.1994. Appl Environ Microbiol. 1994. PMID: 7986047 Free PMC article. - Detection of Campylobacter jejuni added to foods by using a combined selective enrichment and nucleic acid sequence-based amplification (NASBA).
Uyttendaele M, Schukkink R, van Gemen B, Debevere J. Uyttendaele M, et al. Appl Environ Microbiol. 1995 Apr;61(4):1341-7. doi: 10.1128/aem.61.4.1341-1347.1995. Appl Environ Microbiol. 1995. PMID: 7747955 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources