Sestrin as a feedback inhibitor of TOR that prevents age-related pathologies - PubMed (original) (raw)

Jun Hee Lee et al. Science. 2010.

Abstract

Sestrins are conserved proteins that accumulate in cells exposed to stress, potentiate adenosine monophosphate-activated protein kinase (AMPK), and inhibit activation of target of rapamycin (TOR). We show that the abundance of Drosophila sestrin (dSesn) is increased upon chronic TOR activation through accumulation of reactive oxygen species that cause activation of c-Jun amino-terminal kinase and transcription factor Forkhead box O (FoxO). Loss of dSesn resulted in age-associated pathologies including triglyceride accumulation, mitochondrial dysfunction, muscle degeneration, and cardiac malfunction, which were prevented by pharmacological activation of AMPK or inhibition of TOR. Hence, dSesn appears to be a negative feedback regulator of TOR that integrates metabolic and stress inputs and prevents pathologies caused by chronic TOR activation that may result from diminished autophagic clearance of damaged mitochondria, protein aggregates, or lipids.

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Figures

Fig. 1

Fig. 1

Increased abundance of dSesn upon TOR activation. Larval wing discs of indicated strains were stained to visualize indicated proteins or mRNA. The dorsal side points upwards. Dorsoventral boundary (D/V in A) was visualized by staining with an antibody to the wingless (Wg) protein (red). (A to C) Expression of dSesn protein (green) in the absence (A) or presence of InRCA in WT (B) and _dSesn_-null (C) strains. (D and E) Accumulation of dSesn mRNA (green) in response to InRCA detected by in situ hybridization. (F) The signaling network controlling TOR activity and expression of dSesn. (G to K) Accumulation of dSesn (green) in response to Rheb (G) but not S6KCA (J) or loss of 4E-BP (K). Thor1 is a Drosophila 4E-BP loss-of-function mutant. (H and I) Accumulation of dSesn after somatic loss of PTEN (H) or TSC1 (I). Absence of GFP (green) indicates loss of PTEN or TSC1 resulting in dSesn (red) accumulation.

Fig. 2

Fig. 2

Chronic TOR activation results in accumulation of ROS and dSesn. Larval imaginal discs of indicated strains were stained as indicated. (A) ROS accumulation (red) in response to InRCA or Rheb overexpressed in dorsal (upwards) wing discs was revealed by DHE staining. (B) InRCA-induced ROS (red) accumulation in eye discs was reduced by PI3KDN or TORDN but not by S6KDN or 4E-BPCA. (C) DHE staining (red) in TSC1-negative wing disc clones marked by absence of GFP. (D and E) Inhibition of InRCA-induced accumulation of dSesn (green) in eye and wing discs by expression of catalase or peroxiredoxin (Prx). D-V wing boundary and differentiated eye area were visualized by Wg (red) and Elav (red) staining, respectively. (F) dSesn accumulation (red) in TSC1-negative wing disc clones was suppressed by vitamin E feeding. Absence of GFP (green) indicates loss of TSC1. (G) Diagram depicting TOR-stimulated production of ROS and expression of dSesn.

Fig. 3

Fig. 3

Antagonism of TOR-stimulated growth by dSesn. (A to F) Light (left) and scanning electron (right) micrographs of eyes expressing the indicated genetic elements driven by gmr-GAL4. Scale bar, 20 μm. (G) Quantification of eye and ommatidia sizes measured from frontal and lateral views, respectively. P values were calculated by one-way ANOVA. Error bars=S.D.; n=3 and 5, respectively. (H) Suppression of TOR signaling by dSesn. Adult heads with eye-specific expression of indicated genetic elements driven by _gmr_-GAL4 were subjected to immunoblot analyses with indicated antibodies. Relative band intensities were quantified and are presented as bar graphs. Error bars=S.D. n=3. (I to N) Suppression of InR-induced growth by dSesn. Anterior views of wing blades with _apterous-GAL4_-driven expression of indicated genetic elements. Dorsal sides point upwards. (O) Schematic diagram summarizing genetic interactions between dSesn and TOR signaling components.

Fig. 4

Fig. 4

Effect of dSesn on lipid homeostasis. (A) Lipid accumulation in fat bodies examined by Nile Red staining (red). (B) Total triglycerides were measured in five 10-day-old adult males of the indicated genotypes subjected to the indicated treatments (met., metformin; rapa., rapamycin). P values were calculated by one-way ANOVA. Error bars=S.D.; n≥3. (C and D) Protein lysates from fat bodies were analyzed by immunoblotting with indicated antibodies. Relative band intensities were quantified and are shown as a bar graph. Error bars=S.D.; n=3. (E) Expression of indicated mRNAs in adult flies was examined by quantitative RT-PCR. Fifty 3-day-old adult males of each genotype were used to prepare total RNA. Error bars=S.D.; n=3.

Fig. 5

Fig. 5

Effect of dSesn on cardiac function. (A, B, E to G) Representative M mode records of indicated 2-week-old flies fed without or with indicated drugs, showing movement of heart tube walls (y-axis) over time (x-axis). Diastolic (orange) and systolic (blue) diameters are indicated. 1 second is indicated as a bar. (C and D) Quantification of cardiac function parameters. P values were calculated using one-way ANOVA. Error bars indicate S.E.M.; n>10. (H and I) Actin fibers in WT and _dSesn_-null hearts were visualized by phalloidin staining (red).

Fig. 6

Fig. 6

Effect of dSesn on progressive muscle degeneration. Thoracic skeletal muscles of indicated 20-day-old male flies treated without or with indicated drugs were analyzed by transmission electron microscopy (TEM). Left panels: sarcomeres; right panels: mitochondria. Mitochondrial microstructure is shown in the insets (0.15 μm width). Scale bars, 0.2 μm.

Fig. 7

Fig. 7

Phenotypes caused by silencing of dATG1. (A and B) Representative M mode records of control and _dATG1RNAi_-expressing hearts from 2-week-old adult flies, showing the movement of heart tube walls (y-axis) over time (x-axis). Diastolic (orange) and systolic (blue) diameters are indicated. 1 second is indicated as a bar. (C) Quantification of cardiac function parameters. P values were calculated using one-way ANOVA. Error bars indicate S.E.M.; n≥9. (D) _dATG1RNAi_-expressing thoracic skeletal muscle was analyzed by TEM. Left panel: sarcomeres; right panel: mitochondria. Mitochondrial microstructure is shown in the insets (0.15 μm width). Scale bars, 0.2 μm. (E) ROS accumulation (red) in response to dATG1RNAi expressed in dorsal (upwards) wing discs revealed by DHE staining.

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