FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans - PubMed (original) (raw)

FLOTAC: new multivalent techniques for qualitative and quantitative copromicroscopic diagnosis of parasites in animals and humans

Giuseppe Cringoli et al. Nat Protoc. 2010 Mar.

Abstract

Accurate diagnosis of parasitic infections is of pivotal importance for both individual patient management and population-based studies, such as drug efficacy trials and surveillance of parasitic disease control and elimination programs, in both human and veterinary public health. In this study, we present protocols for the FLOTAC basic, dual and double techniques, which are promising new multivalent, sensitive, accurate and precise methods for qualitative and quantitative copromicroscopic analysis. These various methods make use of the FLOTAC apparatus, a cylindrical device with two 5-ml flotation chambers, which allows up to 1 g of stool to be prepared for microscopic analysis. Compared with currently more widely used diagnostic methods for parasite detection in animals (e.g., McMaster and Wisconsin techniques) and humans (e.g., Kato-Katz and ether-based concentration techniques), the FLOTAC techniques show higher sensitivity and accuracy. All FLOTAC techniques can be performed on fresh fecal material as well as preserved stool samples, and require approximately 12-15 min of preparation time before microscopic analysis.

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References

    1. Parassitologia. 2006 Sep;48(3):381-4 - PubMed
    1. Trans R Soc Trop Med Hyg. 2009 Apr;103(4):347-54 - PubMed
    1. Am J Trop Med Hyg. 1996 Apr;54(4):319-24 - PubMed
    1. Am J Trop Med Hyg. 1968 May;17(3):382-91 - PubMed
    1. Am J Trop Med Hyg. 1997 Nov;57(5):571-7 - PubMed

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