MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC) - PubMed (original) (raw)
Comparative Study
. 2010 Jun 3;411(11-12):846-52.
doi: 10.1016/j.cca.2010.02.074. Epub 2010 Mar 16.
Affiliations
- PMID: 20223231
- DOI: 10.1016/j.cca.2010.02.074
Comparative Study
MicroRNA-21 (miR-21) represses tumor suppressor PTEN and promotes growth and invasion in non-small cell lung cancer (NSCLC)
Ji-guang Zhang et al. Clin Chim Acta. 2010.
Abstract
Background: MicroRNAs (miRNAs) are a class of small non-coding RNAs regulating gene expression that play roles in the pathogenesis of human diseases, including malignancy. miR-21, a commonly overexpressed miRNA in very diverse types of malignancies, may affect tumor progression through targeting tumor suppressor genes. We identified the role of miR-21 in non-small cell lung cancer (NSCLC) and to clarify the regulation of PTEN by miR-21 and determine mechanisms of this regulation.
Methods: Expression of miR-21 and PTEN in 20 paired NSCLC and adjacent non-tumor lung tissues was investigated by qRT-PCR and western blot, respectively. The effect of miR-21 on PTEN expression was assessed in NSCLC cell lines with miR-21 inhibitor to decrease miR-21 expression. Furthermore, the roles of miR-21 in cell growth and invasion were analyzed with miR-21 inhibitor-transfected cells.
Results: miR-21 was overexpressed in tumor tissues relative to adjacent non-tumor tissues. Notably, patients with advanced clinical TNM stage (n=16) or distal metastasis (n=5) demonstrated higher miR-21 expression than those without them (n=26, or n=37) (p<0.05, or p<0.001). Tumor tissues showed an inverse correlation between miR-21 and PTEN protein. miR-21 inhibitor transfection increased a luciferase-reporter activity containing the PTEN-3'-UTR construct and increased PTEN protein but not PTEN-mRNA levels in NSCLC cell lines. Finally, miR-21 inhibitor-transfected cells exhibited markedly reduced cell growth and invasive characteristics.
Conclusions: miR-21 post-transcriptionally down-regulates the expression of tumor suppressor PTEN and stimulates growth and invasion in NSCLC. It may be a potential therapeutic target for NSCLC.
Copyright 2010 Elsevier B.V. All rights reserved.
Similar articles
- miR-106a promotes growth and metastasis of non-small cell lung cancer by targeting PTEN.
Xie X, Liu HT, Mei J, Ding FB, Xiao HB, Hu FQ, Hu R, Wang MS. Xie X, et al. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3827-34. eCollection 2015. Int J Clin Exp Pathol. 2015. PMID: 26097565 Free PMC article. - MicroRNA-92a promotes epithelial-mesenchymal transition through activation of PTEN/PI3K/AKT signaling pathway in non-small cell lung cancer metastasis.
Lu C, Shan Z, Hong J, Yang L. Lu C, et al. Int J Oncol. 2017 Jul;51(1):235-244. doi: 10.3892/ijo.2017.3999. Epub 2017 May 16. Int J Oncol. 2017. PMID: 28534966 - miR-205 promotes the growth, metastasis and chemoresistance of NSCLC cells by targeting PTEN.
Lei L, Huang Y, Gong W. Lei L, et al. Oncol Rep. 2013 Dec;30(6):2897-902. doi: 10.3892/or.2013.2755. Epub 2013 Sep 30. Oncol Rep. 2013. PMID: 24084898 - MiR-132 promotes the proliferation, invasion and migration of human pancreatic carcinoma by inhibition of the tumor suppressor gene PTEN.
Zhang H, Liu A, Feng X, Tian L, Bo W, Wang H, Hu Y. Zhang H, et al. Prog Biophys Mol Biol. 2019 Nov;148:65-72. doi: 10.1016/j.pbiomolbio.2017.09.019. Epub 2017 Sep 20. Prog Biophys Mol Biol. 2019. PMID: 28941804 Review. - MicroRNAs in non-small cell lung cancer invasion and metastasis: from the perspective of the radiation oncologist.
Verma V, Lautenschlaeger T. Verma V, et al. Expert Rev Anticancer Ther. 2016 Jul;16(7):767-74. doi: 10.1080/14737140.2016.1191950. Epub 2016 Jun 1. Expert Rev Anticancer Ther. 2016. PMID: 27215143 Review.
Cited by
- p85α is a microRNA target and affects chemosensitivity in pancreatic cancer.
Toste PA, Li L, Kadera BE, Nguyen AH, Tran LM, Wu N, Madnick DL, Patel SG, Dawson DW, Donahue TR. Toste PA, et al. J Surg Res. 2015 Jun 15;196(2):285-293. doi: 10.1016/j.jss.2015.02.071. Epub 2015 Mar 6. J Surg Res. 2015. PMID: 25846727 Free PMC article. - Downregulation of HMGB1 by miR-34a is sufficient to suppress proliferation, migration and invasion of human cervical and colorectal cancer cells.
Chandrasekaran KS, Sathyanarayanan A, Karunagaran D. Chandrasekaran KS, et al. Tumour Biol. 2016 Oct;37(10):13155-13166. doi: 10.1007/s13277-016-5261-1. Epub 2016 Jul 25. Tumour Biol. 2016. PMID: 27456356 - A Perspective of Epigenetic Regulation in Radiotherapy.
Peng Q, Weng K, Li S, Xu R, Wang Y, Wu Y. Peng Q, et al. Front Cell Dev Biol. 2021 Feb 18;9:624312. doi: 10.3389/fcell.2021.624312. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 33681204 Free PMC article. Review. - Clinical assessment of PTEN loss in endometrial carcinoma: immunohistochemistry outperforms gene sequencing.
Djordjevic B, Hennessy BT, Li J, Barkoh BA, Luthra R, Mills GB, Broaddus RR. Djordjevic B, et al. Mod Pathol. 2012 May;25(5):699-708. doi: 10.1038/modpathol.2011.208. Epub 2012 Feb 3. Mod Pathol. 2012. PMID: 22301702 Free PMC article. - Non-coding RNAs as theranostics in human cancers.
Redis RS, Berindan-Neagoe I, Pop VI, Calin GA. Redis RS, et al. J Cell Biochem. 2012 May;113(5):1451-9. doi: 10.1002/jcb.24038. J Cell Biochem. 2012. PMID: 22213511 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Research Materials