The roles of the myofibroblast in idiopathic pulmonary fibrosis. Ultrastructural and immunohistochemical features of sites of active extracellular matrix synthesis - PubMed (original) (raw)
. 1991 May;138(5):1257-65.
Affiliations
- PMID: 2024710
- PMCID: PMC1886011
The roles of the myofibroblast in idiopathic pulmonary fibrosis. Ultrastructural and immunohistochemical features of sites of active extracellular matrix synthesis
C Kuhn et al. Am J Pathol. 1991 May.
Abstract
The synthesis of collagen and EIIIA-containing cellular fibronectin in certain forms of pulmonary fibrosis occurs in discrete locations: in the Masson bodies in bronchiolitis obliterans with organizing pneumonia and in focal clusters of fibroblasts (fibroblastic foci) within airspaces in usual interstitial pneumonia. These sites were examined by electron microscopy and immunohistochemistry using antibodies against cytoskeletal markers and extracellular matrix components in biopsies from three patients with bronchiolitis obliterans with organizing pneumonia and four patients with usual interstitial pneumonia. Fibroblasts of both Masson bodies and fibroblastic foci expressed vimentin and alpha smooth muscle actin but not desmin, distinguishing them from true smooth muscle. In both structures fibroblasts with well-formed actin filament bundles were aligned parallel to one another, enmeshed in a matrix of fibronectin-containing fibrils (microtendons) that linked cells and collagen bundles. Similar features characterize the phase of contraction during the healing of skin wounds. This suggests that active contractions of fibroblasts plays a role in the remodeling of the lung in pulmonary fibrosis.
Similar articles
- Rat alveolar myofibroblasts acquire alpha-smooth muscle actin expression during bleomycin-induced pulmonary fibrosis.
Vyalov SL, Gabbiani G, Kapanci Y. Vyalov SL, et al. Am J Pathol. 1993 Dec;143(6):1754-65. Am J Pathol. 1993. PMID: 7504890 Free PMC article. - An immunohistochemical study of architectural remodeling and connective tissue synthesis in pulmonary fibrosis.
Kuhn C 3rd, Boldt J, King TE Jr, Crouch E, Vartio T, McDonald JA. Kuhn C 3rd, et al. Am Rev Respir Dis. 1989 Dec;140(6):1693-703. doi: 10.1164/ajrccm/140.6.1693. Am Rev Respir Dis. 1989. PMID: 2604297 - Factors influencing myofibroblast differentiation during wound healing and fibrosis.
Desmoulière A. Desmoulière A. Cell Biol Int. 1995 May;19(5):471-6. doi: 10.1006/cbir.1995.1090. Cell Biol Int. 1995. PMID: 7640660 Review. - The myofibroblast: paradigm for a mechanically active cell.
Hinz B. Hinz B. J Biomech. 2010 Jan 5;43(1):146-55. doi: 10.1016/j.jbiomech.2009.09.020. Epub 2009 Oct 3. J Biomech. 2010. PMID: 19800625 Review.
Cited by
- Host responses in tissue repair and fibrosis.
Duffield JS, Lupher M, Thannickal VJ, Wynn TA. Duffield JS, et al. Annu Rev Pathol. 2013 Jan 24;8:241-76. doi: 10.1146/annurev-pathol-020712-163930. Epub 2012 Oct 22. Annu Rev Pathol. 2013. PMID: 23092186 Free PMC article. Review. - Targeting the AXL Receptor in Combating Smoking-related Pulmonary Fibrosis.
Yang DC, Gu S, Li JM, Hsu SW, Chen SJ, Chang WH, Chen CH. Yang DC, et al. Am J Respir Cell Mol Biol. 2021 Jun;64(6):734-746. doi: 10.1165/rcmb.2020-0303OC. Am J Respir Cell Mol Biol. 2021. PMID: 33730527 Free PMC article. - Fibroblast morphogenesis on 3D collagen matrices: the balance between cell clustering and cell migration.
da Rocha-Azevedo B, Grinnell F. da Rocha-Azevedo B, et al. Exp Cell Res. 2013 Oct 1;319(16):2440-6. doi: 10.1016/j.yexcr.2013.05.003. Epub 2013 May 9. Exp Cell Res. 2013. PMID: 23664837 Free PMC article. Review. - Low α(2)β(1) integrin function enhances the proliferation of fibroblasts from patients with idiopathic pulmonary fibrosis by activation of the β-catenin pathway.
Xia H, Seeman J, Hong J, Hergert P, Bodem V, Jessurun J, Smith K, Nho R, Kahm J, Gaillard P, Henke C. Xia H, et al. Am J Pathol. 2012 Jul;181(1):222-33. doi: 10.1016/j.ajpath.2012.03.034. Epub 2012 May 27. Am J Pathol. 2012. PMID: 22642910 Free PMC article. - Extracellular matrix molecules: potential targets in pharmacotherapy.
Järveläinen H, Sainio A, Koulu M, Wight TN, Penttinen R. Järveläinen H, et al. Pharmacol Rev. 2009 Jun;61(2):198-223. doi: 10.1124/pr.109.001289. Pharmacol Rev. 2009. PMID: 19549927 Free PMC article. Review.
References
- Q J Med. 1983 Summer;52(207):382-94 - PubMed
- Lab Invest. 1990 Jul;63(1):21-9 - PubMed
- J Cell Biol. 1984 Jun;98(6):2091-106 - PubMed
- Eur J Cell Biol. 1984 May;34(1):137-43 - PubMed
- N Engl J Med. 1985 Jan 17;312(3):152-8 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical