Acute exercise reverses aged-induced impairments in insulin signaling in rodent skeletal muscle - PubMed (original) (raw)

doi: 10.1016/j.mad.2010.03.004. Epub 2010 Mar 20.

Eduardo R Ropelle, Dennys Esper Cintra, Cláudio Teodoro De Souza, Adelino S R da Silva, Juliana C Moraes, Patricia O Prada, José A C de Almeida Leme, Eliete Luciano, Lício A Velloso, José B C Carvalheira, Mario J A Saad

Affiliations

Acute exercise reverses aged-induced impairments in insulin signaling in rodent skeletal muscle

José R Pauli et al. Mech Ageing Dev. 2010 May.

Abstract

The insulin resistance associated with aging is improved by exercise, but the molecular mechanisms of this improvement are not fully understood. We investigated whether the improvement in insulin action, associated with acute exercise in old rats is dependent on the modulation of pIRS-1Ser307, JNK, IkBalpha and PTP-1B. Aging rats were subjected to swimming for two 1.5-h long bouts, separated by a 45min rest period. Sixteen hours after the exercise, the rats were killed and proteins from the insulin signaling pathway were analyzed by immunoblotting. Our results show that the reduction in glucose disappearance rate (Kitt), observed in aged rats, was restored at 16h after exercise. Aging led to an increase in Ser307 phosphorylation of IRS-1, and this was reversed by exercise in the skeletal muscle, in parallel with a reduction in pJNK and IkBalpha degradation. Moreover, aging induced an increase in the expression of PTP-1B and attenuated insulin signaling in the muscle of rats, a phenomenon that was reversed by exercise. Interestingly, the decrease in PTP-1B expression in the muscle of exercised old rats was accompanied by an increase in SIRT1 expression. These results provide new insights into the mechanisms by which exercise restores insulin sensitivity during aging.

Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources