mShal, a subfamily of A-type K+ channel cloned from mammalian brain - PubMed (original) (raw)

mShal, a subfamily of A-type K+ channel cloned from mammalian brain

M D Pak et al. Proc Natl Acad Sci U S A. 1991.

Abstract

We have cloned and expressed a mouse brain cDNA, mShal, that encodes a transient, A-type K+ current. mShal, the vertebrate homolog of the Drosophila Shal gene, defines a distinct subfamily of voltage-gated K+ channels. The Shal deduced proteins are more highly conserved between mouse and Drosophila than other presently known K+ channels. mShal carries a "low-threshold" A-type current with a hyperpolarized steady-state inactivation midpoint. Marked similarity was observed between mShal and its Drosophila homolog, fShal, with regard to voltage sensitivity of activation, macroscopic inactivation, steady-state inactivation, and 4-aminopyridine sensitivity. Sequence conservation for Shal proteins is unusually high at the amino terminus, an area considered important for inactivation. Removal of conserved amino-terminal residues from mShal modifies macroscopic inactivation but the transient nature of the current is preserved. Underlying the very high conservation of mShal and fShal may be a role in the nervous system that is conserved in widely divergent species.

PubMed Disclaimer

Similar articles

Cited by

References

    1. FEBS Lett. 1988 Dec 19;242(1):199-206 - PubMed
    1. Science. 1990 Oct 12;250(4978):276-9 - PubMed
    1. Science. 1989 Feb 17;243(4893):943-7 - PubMed
    1. Science. 1989 Apr 14;244(4901):221-4 - PubMed
    1. J Biol Chem. 1989 May 15;264(14):8230-6 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources