Digoxigenin-labeled probes for the detection of hepatitis B virus DNA in serum - PubMed (original) (raw)

Comparative Study

Digoxigenin-labeled probes for the detection of hepatitis B virus DNA in serum

K J Guo et al. J Clin Microbiol. 1991 Mar.

Abstract

A nonradioactive hybridization assay for the detection of hepatitis B virus (HBV) DNA in serum with a digoxigenin-labeled probe is described. The probe was sensitive, being able to detect 0.25 pg of homologous HBV DNA, equivalent to 7 x 10(4) genome copies. After extraction of DNA from clinical samples, the probe detected HBV DNA in 11 of 12 hepatitis B e antigen-positive sera and did not react with 6 hepatitis B surface antigen-negative sera. This result was comparable to that obtained with a radiolabeled probe. When serum samples were treated by the alkaline denaturation method, some false-positive reactions were apparent with the digoxigenin-labeled probe, although their frequency could be reduced to around 8% by modifying the sample treatment with a centrifugation step. Overall, the sensitivity and specificity of the digoxigenin-labeled probe indicate that it is a viable alternative to the radiolabeled probe for the detection of HBV DNA in serum. The lack of radioactive reagents in the digoxigenin labeling and detection system and its long shelf-life make this system suitable for routine use in laboratories.

PubMed Disclaimer

References

    1. J Virol Methods. 1989 Jun;24(3):335-46 - PubMed
    1. Liver. 1989 Dec;9(6):322-8 - PubMed
    1. Lancet. 1981 Oct 10;2(8250):765-8 - PubMed
    1. Hepatology. 1981 Sep-Oct;1(5):386-91 - PubMed
    1. J Med Virol. 1982;9(1):57-68 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources