Characterization of human tyrosinase ectodomain expressed in Escherichia coli - PubMed (original) (raw)
Characterization of human tyrosinase ectodomain expressed in Escherichia coli
Ji-Na Kong et al. Protein Pept Lett. 2010 Aug.
Abstract
The human tyrosinase ectodomain has been expressed in Escherichia coli as a soluble form and purified by immobilized metal affinity column chromatography. The ectodomain exhibited tyrosinase activities toward the hydroxylation and oxidation reactions. Biochemical properties of the ectodomain appeared to be distinct from those of the human tyrosinase, although common features were retained.
Similar articles
- Expression and characterization of human tyrosinase from a bacterial expression system.
Kong KH, Park SY, Hong MP, Cho SH. Kong KH, et al. Comp Biochem Physiol B Biochem Mol Biol. 2000 Apr;125(4):563-9. doi: 10.1016/s0305-0491(00)00163-2. Comp Biochem Physiol B Biochem Mol Biol. 2000. PMID: 10904869 - Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media.
Wan X, Chai B, Liao Y, Su Y, Ye T, Shen P, Chen X. Wan X, et al. Appl Microbiol Biotechnol. 2009 Feb;82(2):261-9. doi: 10.1007/s00253-008-1742-5. Epub 2008 Oct 18. Appl Microbiol Biotechnol. 2009. PMID: 18931836 - Expression of recombinant mature human tyrosinase from Escherichia coli and exhibition of its activity without phosphorylation or glycosylation.
Chen GH, Chen WM, Huang YC, Jiang ST. Chen GH, et al. J Agric Food Chem. 2012 Mar 21;60(11):2838-43. doi: 10.1021/jf205021g. Epub 2012 Mar 1. J Agric Food Chem. 2012. PMID: 22352397 - A review on spectrophotometric methods for measuring the monophenolase and diphenolase activities of tyrosinase.
García-Molina F, Muñoz JL, Varón R, Rodríguez-López JN, García-Cánovas F, Tudela J. García-Molina F, et al. J Agric Food Chem. 2007 Nov 28;55(24):9739-49. doi: 10.1021/jf0712301. Epub 2007 Oct 25. J Agric Food Chem. 2007. PMID: 17958393 Review. - Substrate specificity of polyphenol oxidase.
McLarin MA, Leung IKH. McLarin MA, et al. Crit Rev Biochem Mol Biol. 2020 Jun;55(3):274-308. doi: 10.1080/10409238.2020.1768209. Epub 2020 May 22. Crit Rev Biochem Mol Biol. 2020. PMID: 32441137 Review.
Cited by
- Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies.
Lai X, Soler-Lopez M, Wichers HJ, Dijkstra BW. Lai X, et al. PLoS One. 2016 Aug 23;11(8):e0161697. doi: 10.1371/journal.pone.0161697. eCollection 2016. PLoS One. 2016. PMID: 27551823 Free PMC article. - Effect of chlorogenic acid on melanogenesis of B16 melanoma cells.
Li HR, Habasi M, Xie LZ, Aisa HA. Li HR, et al. Molecules. 2014 Aug 25;19(9):12940-8. doi: 10.3390/molecules190912940. Molecules. 2014. PMID: 25157464 Free PMC article. - Albinism-causing mutations in recombinant human tyrosinase alter intrinsic enzymatic activity.
Dolinska MB, Kovaleva E, Backlund P, Wingfield PT, Brooks BP, Sergeev YV. Dolinska MB, et al. PLoS One. 2014 Jan 2;9(1):e84494. doi: 10.1371/journal.pone.0084494. eCollection 2014. PLoS One. 2014. PMID: 24392141 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources