Leukotriene B4 is a physiologically relevant endogenous peroxisome proliferator-activated receptor-alpha agonist - PubMed (original) (raw)
Identification of the specific PPAR-α-activating compound. In A, B, C, and D, cells were transfected with the PPAR-α Gal4-luciferase reporter system, treated, and then lysed and analyzed by Dual-Luciferase assay 24 h later. A, transfected CV-1 cells were treated with arachidonic acid (AA), LTA4, LTB4, LTC4, LTD4, LTE4, 5-HETE, 5-oxo-ETE, or 5-HPETE, all at 20 μ
m
. *, p < 0.5 versus DMSO (one-way ANOVA). B, transfected HeLa cells were treated with LTB4 (1–20 μ
m
) or with Wy 14643 (WY, 10 μ
m
). *, p < 0.5 versus DMSO (one-way ANOVA). C, transfected CV-1 cells were treated with the LTA4 hydrolase inhibitors bestatin (10 and 50 μ
m
) or SC22716 (SC; 1 and 5 μ
m
) and LTA4 (10 μ
m
). *, p < 0.5 versus DMSO (one-way ANOVA). D, transfected Jurkat T cells were treated with bestatin (50 μ
m
) or SC22716 (1 and 5 μ
m
) and stimulated with A23187 (5 μ
m
). Some cells were transfected with LTA4 hydrolase siRNA (LTA4 siRNA) or scrambled siRNA control (siRNA ctrl) for 48 h prior to A23187 stimulation. Inset, shown is a Western blot 48 h after transfection with LTA4 hydrolase siRNA. *, p < 0.5 versus DMSO (one-way ANOVA); †, p < 0.5 versus siRNA control (one-way ANOVA). Data are mean values of three independent experiments performed in triplicate. Error bars represent S.E. Western blots were repeated at least three times.