Cohesinopathies, gene expression, and chromatin organization - PubMed (original) (raw)

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Cohesinopathies, gene expression, and chromatin organization

Tania Bose et al. J Cell Biol. 2010.

Abstract

The cohesin protein complex is best known for its role in sister chromatid cohesion, which is crucial for accurate chromosome segregation. Mutations in cohesin proteins or their regulators have been associated with human diseases (termed cohesinopathies). The developmental defects observed in these diseases indicate a role for cohesin in gene regulation distinct from its role in chromosome segregation. In mammalian cells, cohesin stably interacts with specific chromosomal sites and colocalizes with CTCF, a protein that promotes long-range DNA interactions, implying a role for cohesin in genome organization. Moreover, cohesin defects compromise the subnuclear position of chromatin. Therefore, defects in the cohesin network that alter gene expression and genome organization may underlie cohesinopathies.

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Figures

Figure 1.

Figure 1.

The four subunits of the cohesin complex form a ring structure. The manner in which this ring interacts with DNA is a matter of debate. (A) Both Smc1 and Smc3 fold back on themselves to form intramolecular interactions. They associate to form a heterodimer via their hinge domains. (B) The Smc1-Smc3 heterodimer binds to Rad21 and Scc3, forming a ring. The cohesin ring is shown embracing two sister chromatids, drawn as 10-nm fibers, to mediate cohesion. (C) The cohesin ring may encircle a single sister chromatid and interact with a second ring containing the other sister to mediate cohesion. In this model, the DNA could be accommodated as a 30-nm fiber. The interaction between rings could be mediated through Rad21 and Scc3, a model known as the handcuff model.

Figure 2.

Figure 2.

The formation of chromatin loops is dependent on cohesin. (A) Schematic representation of long-distance interactions at the Igf2/H19 locus. Some interactions (blue arrows) occur on both alleles (biallelic), and some interactions are specific to the maternal or paternal allele. Specific CTCF cohesin–binding sites have been identified by 3C. Upstream of the IgF2 locus lies the differentially methylated region (DMR0), which includes a CTCF-binding site (CTCF AD). The centrally conserved DNase I hypersensitive site (CCD) lies between IgF2 and H19. A CTCF-binding region downstream of the H19 locus is denoted as CTCF DS. CTCF AD and CCD interactions occur on both alleles. (B) Looping may differ at the maternal and paternal Igf2/H19 locus. Cohesin and CTCF bind to the imprinting control region (ICR) when it is not methylated. CTCF AD and CCD interactions occur on both alleles. In the paternal allele, CTCF cohesin colocalization results in looping together the CTCF AD, CCD, and CTCF DS. Methylation of ICR causes CTCF DS to remain out of the loop, which in turn may cause activation of the IgF2 locus via interaction with the enhancer. The maternal allele carries an unmethylated copy of ICR that results in interaction between ICR and CTCF DS, which stops activation of IgF2 by the enhancer. (C) Schematic representation of the apolipoprotein locus. The AC2, AR1, and AC3 elements are bound to cohesin as measured by chromatin immunoprecipitation, but CTCF is only found associated with AC2 and AC3. 3C data indicate that the AC2, AR1, and AC3 elements interact with an enhancer in Hep3B cells, and the interaction is dependent on Rad21 and CTCF (Mishiro et al., 2009). The zone of influence of the enhancer element (yellow) is depicted in blue.

Figure 3.

Figure 3.

Cohesin may contribute to subcellular localization of DNA sequences. tDNAs (yellow) cluster near the nucleolus (gray) in a budding yeast nucleus (Thompson et al., 2003). Genes (black) located adjacent to tDNAs can be silenced, and this depends on the proximity to the nucleolus (Wang et al., 2005). Recently, it was shown that strains bearing cohesinopathy mutations in either Eco1 (eco1-W216G) or Scc2 (scc2-D730V) lose tDNA clustering and tRNA gene–mediated silencing (Gard et al., 2009). GAL2 (red) is normally tethered to the nucleolus, but nucleolar morphology and GAL2 tethering is disrupted in the mutant backgrounds, and the induction of GAL2 is increased. Cohesin may contribute to tethering of tDNAs at a particular subcellular location, and this may affect the regulation of neighboring genes. The eco1-W216G mutation also causes defects in telomere clustering.

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