The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins - PubMed (original) (raw)
. 2010 Apr 30;141(3):458-71.
doi: 10.1016/j.cell.2010.04.007. Epub 2010 Apr 22.
Marc Gerauer, Nachiket Vartak, Sebastian Koch, Zhi-Ping Huang, Markos Pechlivanis, Jürgen Kuhlmann, Lucas Brunsveld, Anchal Chandra, Bernhard Ellinger, Herbert Waldmann, Philippe I H Bastiaens
Affiliations
- PMID: 20416930
- DOI: 10.1016/j.cell.2010.04.007
Free article
The palmitoylation machinery is a spatially organizing system for peripheral membrane proteins
Oliver Rocks et al. Cell. 2010.
Free article
Abstract
Reversible S-palmitoylation of cysteine residues critically controls transient membrane tethering of peripheral membrane proteins. Little is known about how the palmitoylation machinery governs their defined localization and function. We monitored the spatially resolved reaction dynamics and substrate specificity of the core mammalian palmitoylation machinery using semisynthetic substrates. Palmitoylation is detectable only on the Golgi, whereas depalmitoylation occurs everywhere in the cell. The reactions are not stereoselective and lack any primary consensus sequence, demonstrating that substrate specificity is not essential for de-/repalmitoylation. Both palmitate attachment and removal require seconds to accomplish. This reaction topography and rapid kinetics allows the continuous redirection of mislocalized proteins via the post-Golgi sorting apparatus. Unidirectional secretion ensures the maintenance of a proper steady-state protein distribution between the Golgi and the plasma membrane, which are continuous with endosomes. This generic spatially organizing system differs from conventional receptor-mediated targeting mechanisms and efficiently counteracts entropy-driven redistribution of palmitoylated peripheral membrane proteins over all membranes.
2010 Elsevier Inc. All rights reserved.
Similar articles
- A palmitoylation cycle dynamically regulates partitioning of the GABA-synthesizing enzyme GAD65 between ER-Golgi and post-Golgi membranes.
Kanaani J, Patterson G, Schaufele F, Lippincott-Schwartz J, Baekkeskov S. Kanaani J, et al. J Cell Sci. 2008 Feb 15;121(Pt 4):437-49. doi: 10.1242/jcs.011916. Epub 2008 Jan 29. J Cell Sci. 2008. PMID: 18230651 - Palmitoylation of membrane proteins (Review).
Charollais J, Van Der Goot FG. Charollais J, et al. Mol Membr Biol. 2009 Jan;26(1):55-66. doi: 10.1080/09687680802620369. Epub 2008 Dec 10. Mol Membr Biol. 2009. PMID: 19085289 Review. - Two separate motifs cooperate to target stathmin-related proteins to the Golgi complex.
Charbaut E, Chauvin S, Enslen H, Zamaroczy S, Sobel A. Charbaut E, et al. J Cell Sci. 2005 May 15;118(Pt 10):2313-23. doi: 10.1242/jcs.02349. Epub 2005 May 3. J Cell Sci. 2005. PMID: 15870110 - Sphingomyelin synthase 2 is palmitoylated at the COOH-terminal tail, which is involved in its localization in plasma membranes.
Tani M, Kuge O. Tani M, et al. Biochem Biophys Res Commun. 2009 Apr 10;381(3):328-32. doi: 10.1016/j.bbrc.2009.02.063. Epub 2009 Feb 20. Biochem Biophys Res Commun. 2009. PMID: 19233134 - Palmitoylation cycles and regulation of protein function (Review).
Baekkeskov S, Kanaani J. Baekkeskov S, et al. Mol Membr Biol. 2009 Jan;26(1):42-54. doi: 10.1080/09687680802680108. Epub 2009 Jan 30. Mol Membr Biol. 2009. PMID: 19169934 Review.
Cited by
- Palmitoylation of MPP1 (membrane-palmitoylated protein 1)/p55 is crucial for lateral membrane organization in erythroid cells.
Łach A, Grzybek M, Heger E, Korycka J, Wolny M, Kubiak J, Kolondra A, Bogusławska DM, Augoff K, Majkowski M, Podkalicka J, Kaczor J, Stefanko A, Kuliczkowski K, Sikorski AF. Łach A, et al. J Biol Chem. 2012 Jun 1;287(23):18974-84. doi: 10.1074/jbc.M111.332981. Epub 2012 Apr 10. J Biol Chem. 2012. PMID: 22496366 Free PMC article. Retracted. - Inhibition of NRAS Signaling in Melanoma through Direct Depalmitoylation Using Amphiphilic Nucleophiles.
Vora HD, Johnson M, Brea RJ, Rudd AK, Devaraj NK. Vora HD, et al. ACS Chem Biol. 2020 Aug 21;15(8):2079-2086. doi: 10.1021/acschembio.0c00222. Epub 2020 Jul 13. ACS Chem Biol. 2020. PMID: 32568509 Free PMC article. - Targeting RAS Membrane Association: Back to the Future for Anti-RAS Drug Discovery?
Cox AD, Der CJ, Philips MR. Cox AD, et al. Clin Cancer Res. 2015 Apr 15;21(8):1819-27. doi: 10.1158/1078-0432.CCR-14-3214. Clin Cancer Res. 2015. PMID: 25878363 Free PMC article. Review. - S-acylation-dependent association of the calcium sensor CBL2 with the vacuolar membrane is essential for proper abscisic acid responses.
Batistič O, Rehers M, Akerman A, Schlücking K, Steinhorst L, Yalovsky S, Kudla J. Batistič O, et al. Cell Res. 2012 Jul;22(7):1155-68. doi: 10.1038/cr.2012.71. Epub 2012 May 1. Cell Res. 2012. PMID: 22547024 Free PMC article. - VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking.
Zhou M, Wiener H, Su W, Zhou Y, Liot C, Ahearn I, Hancock JF, Philips MR. Zhou M, et al. J Cell Biol. 2016 Aug 15;214(4):445-58. doi: 10.1083/jcb.201604061. Epub 2016 Aug 8. J Cell Biol. 2016. PMID: 27502489 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases