Schistosoma mansoni Annexin 2: molecular characterization and immunolocalization - PubMed (original) (raw)
. 2010 Oct;126(2):146-55.
doi: 10.1016/j.exppara.2010.04.008. Epub 2010 Apr 22.
Affiliations
- PMID: 20417203
- DOI: 10.1016/j.exppara.2010.04.008
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Schistosoma mansoni Annexin 2: molecular characterization and immunolocalization
Cibele Aparecida Tararam et al. Exp Parasitol. 2010 Oct.
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Abstract
We here describe the cloning and characterization of the Schistosoma mansoni Annexin 2, previously identified in the tegument by proteomic studies, and as an up-regulated gene in schistosomulum stage by microarray data. In silico analysis predicts a conserved core containing four repeat domains of Annexin (ANX) and a variable N-terminal region similar to that described for mammalian isoforms. Real-time RT-PCR and Western blot analysis determined that S. mansoni Annexin 2 is significantly up-regulated in the transition from free-living cercaria to schistosomulum and adult worm parasitic stages. Immunolocalization experiments and tegument membrane preparations confirmed Annexin 2 as a protein mainly localized in the tegument of schistosomula and adult worms. Furthermore, it binds to the tegument surface membranes in a calcium-dependent manner. These results suggest that S. mansoni Annexin 2 is closely associated to the tegument arrangement, being a potential target for immune intervention.
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