Improvement of the representation of bifidobacteria in fecal microbiota metagenomic libraries by application of the cpn60 universal primer cocktail - PubMed (original) (raw)

Comparative Study

. 2010 Jul;76(13):4550-2.

doi: 10.1128/AEM.01510-09. Epub 2010 Apr 30.

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Comparative Study

Improvement of the representation of bifidobacteria in fecal microbiota metagenomic libraries by application of the cpn60 universal primer cocktail

Janet E Hill et al. Appl Environ Microbiol. 2010 Jul.

Abstract

Actinobacteria, particularly bifidobacteria, are widely observed to be underrepresented in metagenomic studies of microbial communities. We have compared human fecal microbiota clone libraries based on 16S rRNA and cpn60 PCR products. Taxonomic profiles were similar except that the cpn60 libraries contained large numbers of bifidobacterial sequences.

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Figures

FIG. 1.

FIG. 1.

Proportional representation of major taxonomic groups in the PCR product clone libraries generated from three diets, based on either 16S rRNA or cpn60. Each pair of libraries was generated from the same sample pool and DNA extract, as described in the text. cpn60 libraries are shown with and without inclusion of bifidobacterial sequences.

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