The key nickel enzyme of methanogenesis catalyses the anaerobic oxidation of methane - PubMed (original) (raw)
. 2010 Jun 3;465(7298):606-8.
doi: 10.1038/nature09015.
Affiliations
- PMID: 20520712
- DOI: 10.1038/nature09015
The key nickel enzyme of methanogenesis catalyses the anaerobic oxidation of methane
Silvan Scheller et al. Nature. 2010.
Abstract
Large amounts (estimates range from 70 Tg per year to 300 Tg per year) of the potent greenhouse gas methane are oxidized to carbon dioxide in marine sediments by communities of methanotrophic archaea and sulphate-reducing bacteria, and thus are prevented from escaping into the atmosphere. Indirect evidence indicates that the anaerobic oxidation of methane might proceed as the reverse of archaeal methanogenesis from carbon dioxide with the nickel-containing methyl-coenzyme M reductase (MCR) as the methane-activating enzyme. However, experiments showing that MCR can catalyse the endergonic back reaction have been lacking. Here we report that purified MCR from Methanothermobacter marburgensis converts methane into methyl-coenzyme M under equilibrium conditions with apparent V(max) (maximum rate) and K(m) (Michaelis constant) values consistent with the observed in vivo kinetics of the anaerobic oxidation of methane with sulphate. This result supports the hypothesis of 'reverse methanogenesis' and is paramount to understanding the still-unknown mechanism of the last step of methanogenesis. The ability of MCR to cleave the particularly strong C-H bond of methane without the involvement of highly reactive oxygen-derived intermediates is directly relevant to catalytic C-H activation, currently an area of great interest in chemistry.
Similar articles
- Methyl-coenzyme M reductase from methanogenic archaea: isotope effects on the formation and anaerobic oxidation of methane.
Scheller S, Goenrich M, Thauer RK, Jaun B. Scheller S, et al. J Am Chem Soc. 2013 Oct 9;135(40):14975-84. doi: 10.1021/ja406485z. Epub 2013 Sep 25. J Am Chem Soc. 2013. PMID: 24004388 - The reaction mechanism of methyl-coenzyme M reductase: how an enzyme enforces strict binding order.
Wongnate T, Ragsdale SW. Wongnate T, et al. J Biol Chem. 2015 Apr 10;290(15):9322-34. doi: 10.1074/jbc.M115.636761. Epub 2015 Feb 17. J Biol Chem. 2015. PMID: 25691570 Free PMC article. - Probing the reactivity of Ni in the active site of methyl-coenzyme M reductase with substrate analogues.
Goenrich M, Mahlert F, Duin EC, Bauer C, Jaun B, Thauer RK. Goenrich M, et al. J Biol Inorg Chem. 2004 Sep;9(6):691-705. doi: 10.1007/s00775-004-0552-1. Epub 2004 Jun 15. J Biol Inorg Chem. 2004. PMID: 15365904 - Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes.
Thauer RK. Thauer RK. Biochemistry. 2019 Dec 31;58(52):5198-5220. doi: 10.1021/acs.biochem.9b00164. Epub 2019 Apr 5. Biochemistry. 2019. PMID: 30951290 Free PMC article. Review.
Cited by
- The Mechanism of Sodium Sulfate Coupled with Anaerobic Methane Oxidation Mitigating Methane Production in Beef Cattle.
Zhu X, Zhou Z, Cheng Y, Deng Z, Wu H, Nussio LG, Zhou Z, Meng Q. Zhu X, et al. Microorganisms. 2024 Sep 3;12(9):1825. doi: 10.3390/microorganisms12091825. Microorganisms. 2024. PMID: 39338499 Free PMC article. - Toward the Use of Methyl-Coenzyme M Reductase for Methane Bioconversion Applications.
Dinh TA, Allen KD. Dinh TA, et al. Acc Chem Res. 2024 Sep 17;57(18):2746-2757. doi: 10.1021/acs.accounts.4c00413. Epub 2024 Aug 27. Acc Chem Res. 2024. PMID: 39190795 Free PMC article. - Capturing methane with recombinant soluble methane monooxygenase and recombinant methyl-coenzyme M reductase.
Sanchez-Torres V, Wood TK. Sanchez-Torres V, et al. Microb Biotechnol. 2024 Aug;17(8):e70000. doi: 10.1111/1751-7915.70000. Microb Biotechnol. 2024. PMID: 39160605 Free PMC article. Review. - Methyl-reducing methanogenesis by a thermophilic culture of Korarchaeia.
Krukenberg V, Kohtz AJ, Jay ZJ, Hatzenpichler R. Krukenberg V, et al. Nature. 2024 Aug;632(8027):1131-1136. doi: 10.1038/s41586-024-07829-8. Epub 2024 Jul 24. Nature. 2024. PMID: 39048017 - The crystal structure of methanogen McrD, a methyl-coenzyme M reductase-associated protein.
Sutherland-Smith AJ, Carbone V, Schofield LR, Cronin B, Duin EC, Ronimus RS. Sutherland-Smith AJ, et al. FEBS Open Bio. 2024 Aug;14(8):1222-1229. doi: 10.1002/2211-5463.13848. Epub 2024 Jun 14. FEBS Open Bio. 2024. PMID: 38877345 Free PMC article.
References
- Crit Rev Biochem Mol Biol. 2008 Jan-Feb;43(1):63-88 - PubMed
- J Biol Inorg Chem. 2002 Jan;7(1-2):101-12 - PubMed
- Microbiology (Reading). 1998 Sep;144 ( Pt 9):2377-2406 - PubMed
- Nature. 2002 May 30;417(6888):507-14 - PubMed
- Nature. 1999 Apr 29;398(6730):802-5 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous